Resistência de amplo espectro em germoplasma de alface aos patógenos de solo <i>Berkeleyomyces basicola</i> e <i>B. rouxiae</i>

Perdomo, David Nataren; Torres, Tiago Bezerra; Souza, Ruthe Lima de; Cabral, Cléia Santos; Fonseca, Maria Esther de Noronha; Boiteux, Leonardo Silva; Reis, Ailton

doi:10.1590/s0102-0536-2025-e286385

person David Nataren Perdomo

All authors contributed to the design and overall organization of the study · developed the idea and wrote the manuscript in equal parts · help with the lab assays, greenhouse works and inoculation procedures · All authors read, commented and approved the final version of the manuscript

schoolUniversidade Federal Rural de Pernambuco(UFRPE), Programa de Pós-Graduação em Fitopatologia, Recife-PE, Brasil, d.natharen91@gmail.com, tiagob.torres@yahoo.com.br, ruthelimade@gmail.comschoolNational University of Agriculture, Catacamas, 16201, Olancho, Honduras; *Corresponding Author:ailton.reis@embrapa.br

0000-0002-0259-096X

person Tiago Bezerra Torres

All authors contributed to the design and overall organization of the study · developed the idea and wrote the manuscript in equal partshelp with the lab assays, greenhouse works and inoculation procedures · All authors read, commented and approved the final version of the manuscript

schoolUniversidade Federal Rural de Pernambuco(UFRPE), Programa de Pós-Graduação em Fitopatologia, Recife-PE, Brasil, d.natharen91@gmail.com, tiagob.torres@yahoo.com.br, ruthelimade@gmail.com

0000-0001-8017-2977

person Ruthe Lima de Souza

All authors contributed to the design and overall organization of the study · help with the lab assays · works and inoculation procedures · All authors read, commented and approved the final version of the manuscript

schoolUniversidade Federal Rural de Pernambuco(UFRPE), Programa de Pós-Graduação em Fitopatologia, Recife-PE, Brasil, d.natharen91@gmail.com, tiagob.torres@yahoo.com.br, ruthelimade@gmail.com

0000-0001-7928-6109

person Cléia Santos Cabral

All authors contributed to the design and overall organization of the study · help with the lab assays, greenhouse works and inoculation procedures · All authors read, commented and approved the final version of the manuscript

schoolUPIS Campus 2, Brasília-DF, Brasil, clea.86@gmail.com

0000-0003-1316-367X

person Maria Esther de Noronha Fonseca

All authors contributed to the design and overall organization of the study · help with the lab assays · greenhouse works · inoculation procedures · All authors read, commented and approved the final version of the manuscript

schoolEmbrapa Vegetable Crops (Embrapa Hortaliças), National Center for Vegetable Crops Research (CNPH), Brasília-DF, Brasil, maria.boiteux@embrapa.br, leonardo.boiteux@embrapa.br

0000-0002-0180-5594

person Leonardo Silva Boiteux

All authors contributed to the design and overall organization of the study · developed the idea and wrote the manuscript in equal parts · help with the lab assays · greenhouse works and inoculation procedures · All authors read, commented and approved the final version of the manuscript

schoolUniversidade Federal Rural de Pernambuco(UFRPE), Programa de Pós-Graduação em Fitopatologia, Recife-PE, Brasil, d.natharen91@gmail.com, tiagob.torres@yahoo.com.br, ruthelimade@gmail.comschoolEmbrapa Vegetable Crops (Embrapa Hortaliças), National Center for Vegetable Crops Research (CNPH), Brasília-DF, Brasil, maria.boiteux@embrapa.br, leonardo.boiteux@embrapa.br

0000-0002-5791-8002

person Ailton Reis

All authors contributed to the design and overall organization of the study · developed the idea and wrote the manuscript in equal parts · All authors read, commented and approved the final version of the manuscript

schoolUniversidade Federal Rural de Pernambuco(UFRPE), Programa de Pós-Graduação em Fitopatologia, Recife-PE, Brasil, d.natharen91@gmail.com, tiagob.torres@yahoo.com.br, ruthelimade@gmail.comschoolEmbrapa Vegetable Crops (Embrapa Hortaliças), National Center for Vegetable Crops Research (CNPH), Brasília-DF, Brasil, maria.boiteux@embrapa.br, leonardo.boiteux@embrapa.br

0000-0002-5705-3002

Conflicts of interest

Conflicts of interest: The authors declare that there is no conflict of interest.

Accessions	Morphotype	Disease reaction		Severity index (%)³
Accessions	Morphotype	Grade¹	Class²	Severity index (%)³
BRS Mediterrânea	CGL	1.42	R	28.33 a⁴
La Brillante	BAT	1.42	R	28.33 a
Flashy Trouts Back	ROM	1.50	R	30.00 a
Litte Gem PI 617959	ROM	1.50	R	30.00 a
BRS Lélia	CGL	1.56	R	31.66 a
Romana Balão	ROM	1.58	R	31.66 a
Crespa Itapuã Super	CGL	1.60	R	31.66 a
Argeles	CGL	1.75	R	35.00 a
Maravilha 4 Estações	BHD	1.75	R	35.00 a
Salinas 88	ICE	1.75	R	35.00 a
Salvius	ROM	1.75	R	35.00 a
Vitoria de Santo Antão	BHD	1.75	R	35.00 a
Prado Mimosa	MIM	1.82	R	36.66 a
Balesta	CGL	1.83	R	36.66 a
Betania	CPL	1.83	R	36.66 a
NUM DM 17	CGL	1.83	R	36.66 a
Veneranda	CGL	1.83	R	36.66 a
Crespa Repolhuda	CGL	1.92	R	38.33 a
Crespa Verão	CGL	1.92	R	38.33 a
Hanson	CGL	1.92	R	38.33 a
BRS Leila	CGL	1.92	R	38.33 a
Penlake PI 536753	ROM	1.92	R	38.33 a
Simpson	CGL	1.92	R	38.33 a
Valmaine PI 543959	ROM	1.92	R	38.33 a
Blonde de Paris	BAT	2.00	R	40.00 a
Crespa Verde	CGL	2.00	R	40.00 a
Joker	CPL	2.00	R	40.00 a
Salad Bowl	MIM	2.00	R	40.00 a
Regina de Verão	BHD	2.00	R	40.00 a
Rubi Crespa	CPL	2.00	R	40.00 a
UCO 7107	MIM	2.00	R	40.00 a
Bourguignonne	BHD	2.08	I	41.66 a
Fenke	BHD	2.08	I	41.66 a
Lednicky PI 674756	ICE	2.08	I	41.66 a
Babá de Verão	BHD	2.17	I	43.33 a
UCO 7105	CGL	2.17	I	43.33 a
Ninja	CGL	2.25	I	45.00 a
Versaii	BHD	2.25	I	45.00 a
CGDM 16	ICE	2.33	I	46.67 a
Hilde	BHD	2.33	I	46.67 a
Pavane PI 667705	ICE	2.42	I	48.33 a
Tiffany	ICE	2.42	I	48.33 a
Sem Rival	BHD	2.58	I	51.67 b
Grand Rapids TBR	CGL	2.60	I	51.67 b
Iceberg	ICE	2.67	I	53.33 b
Belford	ICE	2.67	I	53.33 b
Capitan	BHD	2.75	I	55.00 b
Green Towers PI 601336	ROM	2.75	I	55.00 b
Vanda	CGL	2.83	I	56.67 b
Gallega de Invierno	BHD	2.90	I	58.33 b
Crocantela	CGL	3.00	I	60.00 b
Patriot	ROM	3.00	I	60.00 b
Samira	CGL	3.00	I	60.00 b
Grand Rapids	CGL	3.08	I	61.66 b
Great Lakes 659	ICE	3.08	I	61.66 b
Vanguard 75 PI 536852	ICE	3.08	I	61.66 b
Sabine	ICE	3.10	I	61.66 b
Ithaca PI 536844	ICE	3.17	I	63.33 b
UCO 2206	CGL	3.17	I	63.33 b
R4T57D	CGL	3.25	I	65.00 b
RYZ 2164	ICE	3.25	I	65.00 b
Cohban Green PI 612637	ICE	3.40	I	68.33 b
Hilde II	BHD	3.42	I	68.33 b
Aurélia	BHD	4.08	S	81.67 c
Dandie	ICE	4.08	S	81.67 c
Vanguard PI 536812	ICE	4.08	S	81.67 c
Branca de Paris	ROM	4.17	S	83.33 c
Elisa	BHD	4.25	S	85.00 c
CV	19.75

Accession	Morphotype*	Disease severity index/isolate¹				Mean
Accession	Morphotype*	EH-2733	EH-2740	EH-2741	EH-2743
Litte Gem (PI 617959)	ROM	30.00 Aa	20.00 Aa	30.00 Aa	30.00 Aa2	27.50
Maravilha 4 Estações	BHD	23.33 Aa	26.67 Aa	30.00 Aa	30.00 Aa	27.50
Salvius	ROM	33.33 Aa	26.67 Aa	23.33 Aa	26.67 Aa	27.50
Argeles	CGL	33.33 Aa	30.00 Aa	30.00 Aa	23.33 Aa	29.17
La Brillante	BAT	30.00 Aa	26.67 Aa	30.00 Aa	30.00 Aa	29.17
Balesta	CGL	20.00 Aa	26.67 Aa	30.00 Aa	43.33 Ab	30.00
Betânia	CPL	30.00 Aa	30.00 Aa	23.33 Aa	40.00 Ab	30.83
Branca de Paris	ROM	30.00 Aa	30.00 Aa	23.33 Aa	40.00 Ab	30.83
Joker	CPL	30.00 Aa	30.00 Aa	30.00 Aa	40.00 Ab	32.50
NUM DM 17	CGL	33.33 Aa	30.00 Aa	46.67 Ab	23.33 Aa	33.33
Vitoria de Santo Antão	BHD	33.33 Aa	23.33 Aa	23.33 Aa	60.00 Ab	35.00
Crespa Repolhuda	CGL	33.33 Aa	26.67 Aa	23.33 Aa	66.67 Bc	37.50
Valmaine (PI 543959)	ROM	36.67 Aa	26.67 Aa	36.67 Ab	46.67 Ab	36.66
Flashy Trouts Back	ROM	36.67 Aa	46.67 Aa	26.67 Aa	43.33 Ab	38.33
Veneranda	CGL	40.00 Aa	30.00 Aa	46.67 Ab	33.33 Aa	37.50
Hanson	CGL	30.00 Aa	23.33 Aa	63.33 Bc	50.00 Bb	41.66
Rubi Crespa	CPL	53.33 Ab	43.33 Aa	33.33 Aa	33.33 Aa	40.83
BRS Mediterrânea	CGL	36.67 Aa	33.33 Aa	30.00 Aa	70.00 Bc	42.50
Salinas 88	ICE	30.00 Aa	33.33 Aa	66.67 Bc	40.00 Ab	42.50
Crespa Itapuã Super	CGL	56.67 Ab	40.00 Aa	43.33 Ab	46.67 Ab	46.67
PI 342444	BHD	40.00 Aa	30.00 Aa	70.00 Bc	50.00 Bb	47.50
Vanguard 75 (PI 536812)	ICE	70.00 Bc	43.33 Aa	60.00 Bc	26.67 Aa	50.00
Prado Mimosa	MIM	40.00 Aa	33.33 Aa	60.00 Bc	70.00 Bc	50.83
Salad Bowl	MIM	33.33 Aa	40.00 Aa	46.67 Ab	83.33 Bd	50.83
Crespa Verde	CGL	43.33 Aa	66.67 Bb	66.67 Bc	43.33 Ab	55.00
Simpson	CGL	40.00 Aa	50.00 Aa	53.33 Ac	100.00 Bd	60.83
BRS Lélia	CGL	43.33 Ba	23.33 Aa	93.33 Cd	90.00 Cd	62.50
Crespa Verão	CGL	40.00 Aa	33.33 Aa	80.00 Bd	100.00 Bd	63.33
UCO 7107	MIM	53.33 Bb	33.33 Aa	66.67 Bc	96.67 Cd	62.50
BRS Leila	CGL	63.33 Bb	70.00 Bb	83.33 Bd	46.67 Ab	65.83
Aurélia	BHD	76.66 Ac	56.67 Ab	70.00 Ac	83.33 Ad	71.67
Elisa	BHD	76.66 Ac	100.00 Ac	90.00 Ad	93.33 Ad	90.00
Regina de Verão	BHD	90.00 Ac	100.00 Ac	96.67 Ad	83.33 Ad	92.50
Mean	- - - - -	42.12	38.89	49.29	54.04
CV	- - - - -	20.25

[TFN1] 1Mean disease reaction according to a rating scale ranging from 1 to 5. 2Disease reaction categories: Resistant (R), Intermediate (I), Susceptible (S) according to Sala et al. (2003). 3Disease severity, calculated by the McKinney index (1923), using the frequencies of disease classes considering a scale of grades from 1 to 5. 4Means followed by the same letter in the column do not differ significantly from each other by the Scott-Knott test (P<0.05). **To obtain the letters and CV, the disease index was transformed by the square root of X+1. *Lettuce morphotypes: Crispy Green Loose-Leaf (CGL), Crispy Purple Loose-Leaf (CPL), Iceberg (ICE), Butterhead (BHD), Cos/Romaine (ROM) Mimosa (MIM) and Batavian (BAT).

Resistência de amplo espectro em germoplasma de alface aos patógenos de solo Berkeleyomyces basicola e B. rouxiae