Larvas de <i>Pseudoproleptus</i> sp. (Nematoda: Cystidicolidae) parasitando <i>Macrobrachium amazonicum</i> (Decapoda: Crustacea) no estado do Pará, Norte do Brasil

Cardoso, Patrick José Colares; Cristo, Liane Rodrigues Galvão de; Santa Brigida, Yan Rafael Gillet; Giese, Elane Guerreiro; Pinheiro, Raul Henrique da Silva

doi:10.1590/S1984-29612025042

person Patrick José Colares Cardoso

conceived · designed the study · collected the samples · performed the laboratory analysis · Reviewed and approved the final version of the manuscript

schoolLaboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

person Liane Rodrigues Galvão de Cristo

collected the samples · performed the laboratory analysis · Reviewed and approved the final version of the manuscript

schoolLaboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

person Yan Rafael Gillet Santa Brigida

analyzed the data · Reviewed and approved the final version of the manuscript

schoolLaboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

person Elane Guerreiro Giese

drafted the manuscript · Reviewed and approved the final version of the manuscript

schoolLaboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, BrasilschoolPrograma de Pós-graduação em Saúde e Produção Animal na Amazônia, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

person Raul Henrique da Silva Pinheiro *

conceived · designed the study · analyzed the data · Reviewed and approved the final version of the manuscript

schoolLaboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, BrasilschoolPrograma de Pós-graduação em Saúde e Produção Animal na Amazônia, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia – UFRA, Belém, PA, Brasil

0000-0003-3221-5017

person*Corresponding author: Raul Henrique da Silva Pinheiro. Endereço: Universidade Federal Rural da Amazônia: Avenida Presidente Tancredo Neves, Nº 2501 Bairro: Terra Firme Cep: 66.077-830 Cidade: Belém-Pará-Brasil. Telefone: 91 988481597 Email:
procamallanus@gmail.com https://orcid.org/0000-0003-3221-5017

Assistant Editor:

Marcos Tavares Dias

Conflict of interest

The authors declare that they have no conflict of interest.

Figuras (3)
Tabelas (4)

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Figure 1 Third-stage larva of Pseudoproleptus sp. from Macrobrachium amazonicum, commercialized in Pará state, northern coast of Brazil: (A) Anterior lateral view, cephalic helmet-like cuticular structure (arrowhead), nerve ring (nr), muscular esophagus (mo) and excretory pore (ep). Bar = 100 μm; (B) Detail of prostom (pr), vestibule (ve) and deirids (arrowheads). Bar = 50 μm; (C) Posterior portion, portion of the intestine (in), rectum (r), unicellular rectal glands (*), anus (an), the tail with mucron (mu). Bar = 50 μm.

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Figure 2 Third-stage larva of Pseudoproleptus sp. from Macrobrachium amazonicum, SEM micrographs: (A) Cephalic apical view: amphid (a), cephalic papilla of internal circle (pi); cephalic papilla of external circle (pe), cephalic helmet-like cuticular structure (cc), pseudolabium (pl), pseudolabial terminal protrusion (*), outer, elongate part of sublabium (arrowhead). Bar = 10 μm; (B) Anterior end of body, lateral view cephalic helmet-like cuticular structure. Bar = 10 μm; (C) Deirid views. Bar = 2 μm; (D) Excretory pore. Bar = 10 μm; (E) Tail, lateral view, with small knob-like terminal projection (arrowhead). Bar = 20 μm.

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Figure 3 Histograms showing the total length classes of male (A) and female (B) Macrobrachium amazonicum.

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Table 1 Descriptive statistics of biometric parameters of Macrobrachium amazonicum collected in Guamá River, Pará state, northern Brazil.

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Table 2 Parasitological indices of larvae of Pseudoproleptus sp. harvested from Macrobrachium amazonicum from Guamá River, in Pará state northern Brazil.

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Table 3 Results of the t-test and Mann-Whitney of biometric parameters between, males, females, parasitized and parasite-free shrimps.

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Table 4 Morphological and morphometric comparison of third-stage larvae of Pseudoproleptus sp. in Macrobrachium amazonicum from Guamá River, in the state of Pará, Brazil.

Sex	Biometric parameter	Min.	Max.	x̄	±SD	Median
♀+♂	TL (cm)	3.39	8.91	6.34	1.13	6.26
	CL (cm)	1.38	4.49	2.80	0.58	2.73
	AL (cm)	2.01	4.9	3.55	0.63	3.56
	Weight (g)	0.64	5.7	2.41	1.05	2.27
♂	TL (cm)	3.39	8.63	6.35	1.17	6.31
	CL (cm)	1.38	3.94	2.80	0.55	2.73
	AL (cm)	2.01	4.9	3.55	0.67	3.64
	Weight (g)	0.64	5.7	2.41	1.06	2.29
♀	TL (cm)	4.35	8.91	6.34	1.08	6.22
	CL (cm)	1.71	4.49	2.79	0.61	2.75
	AL (cm)	2.3	4.81	3.55	0.58	3.55
	Weight (g)	0.8	5.02	2.42	1.04	2.07

Sex	P (%)	MI	±SD	MA	±SD	R
♀+♂	15.8	3.9	4.85	0.6	2.36	1–21
♀	14.0	5.8	7.47	0.8	3.32	1–21
♂	18.0	2.7	2.05	0.5	1.32	1–7

	TL (cm)			CL (cm)			Weight (g)
	t	p	CI	t	p	CI	w	p	CI
♂_f × ♂_i	-3.1	0.01	[-1.4584, -0.2896]	-2.01	0.06	[-0.70525, 0.0189]	270	0.23	[-1.2390, 0.1999]
♀_f × ♀_i	-0.09	0.93	[-0.6442, 0.5935]	-0.07	0.94	[-0.33076, 0.3083]	152	0.98	[-0.7800, 0.9200]
♂ × ♀	-0.03	0.98	[-0.4152, 0.4044]	-0.07	0.94	[-0.2238, 0.2077]	1771	0.91	[-0.3900, 0.3599]
♀♂_f × ♀♂_i	-2.53	0.02	[-0.9971, -0.1094]	-1.76	0.08	[-0.47142, 0.0353]	823	0.33	[-0.8000, 0.2401]

Caracteres	*Third-stage larvae of Pseudoproleptus* spp.**
Group of host	Decapoda		Cichliformes	Siluriformes
Family	Palaemonidae		Cichlidae	Auchenipteridae
Hosts	*Macrobrachium amazonicum*		*Satanoperca jurupari*	*Ageneiosus* *ucayalensis*
Locality	Guamá River, state of Pará	Mexiana Island, state of Pará	Guamá River, state of Pará
Prevalence	-	32%	0.90%	0.90%
Length ^mm	17‒28	19.71‒25.65	16.20‒21.63	20
Width	143‒229	163‒190	140‒180	96
Cephalic helmet-like ^L	20‒32	‒	30‒50	‒
Cephalic helmet-like ^W	45‒93	‒	45‒85	‒
Vestibule with prostom ^L	120‒193	165‒189	112‒175	125
Deirids	110‒167	174‒195	110‒200	‒
Nerve ring	165‒257	249‒282	180‒285	‒
Excretory pore	340‒557	530‒558	412‒482	‒
Muscular esophagus ^{L, mm}	0.829‒1.2	0.804‒0.930	0.640‒1	1.053
Muscular esophagus ^W	12‒35	15‒21	20‒50	‒
Glandular esophagus ^{L, mm}	3‒4	3.66‒4.27	3.20‒3.90	4.03
Glandular esophagus ^W	47‒83	57‒66	50‒70	‒
Tail	102‒163	135‒180	100‒145	‒
Mucron	5‒8	3‒6	‒	‒
References	From this study	Moravec & Santos (2009)	Melo et al. (2011)

[1] TL: Total length; CL: Cephalothorax length; AL: Abdominal length. ♀: Female; ♂: Male. Min.: minimum values; Max.: maximum values. x̅ = mean; ±SD = standard deviation.

Larvas de Pseudoproleptus sp. (Nematoda: Cystidicolidae) parasitando Macrobrachium amazonicum (Decapoda: Crustacea) no estado do Pará, Norte do Brasil