Molecular differentiation between <i>Plinia cauliflora</i> (Mart.) Kausel and <i>Plinia trunciflora </i>(O. Berg) Kausel using nuclear SSR markers

SILVA, ANA KELLY S.; FRITSCHE, YOHAN; BEISE, DALVAN CARLOS; BRAND, INGRID LOHANI D.; DANNER, MOESES A.; CITADIN, IDEMIR; WAGNER JÚNIOR, AMÉRICO; MÜLLER, MARKUS; GAILING, OLIVER; STEFENON, VALDIR MARCOS

doi:10.1590/0001-3765202620250519

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CELLULAR AND MOLECULAR BIOLOGY • An. Acad. Bras. Ciênc. 98 (1) • 2026 • https://doi.org/10.1590/0001-3765202620250519 linkcopy

Molecular differentiation between Plinia cauliflora (Mart.) Kausel and Plinia trunciflora (O. Berg) Kausel using nuclear SSR markers

Authorship SCIMAGO INSTITUTIONS RANKINGS

Molecular markers are important tools for genetic studies, including evolution, species differentiation, and population genetics. The genus Plinia (Myrtaceae) consists of various fruit tree species, some of which are referred to as jaboticaba. The subtle interspecific morphological differences among some jaboticaba species make their taxonomy challenging. This study aimed to develop nuclear SSR markers to distinguish and analyze the genetics of two jaboticaba species, Plinia cauliflora and Plinia trunciflora. The developed markers – eight for P. cauliflora and seven for P. trunciflora – were cross-amplified in both species. Jointly, these markers presented high discriminatory power, private alleles at the species level, and distinguished populations of both species.

Key words
Microsatellite markers; genetic diversity; molecular markers; jaboticaba

Locus	Primer sequence (5’ - 3’)	Motif	Expected allele size (bp)	A$	Ae	Ho	He	F	pA <10%	pA >10%
CAU2	F: TCCTTGGTATCTGGGAGCAC R: ACTACACGCGTTGAGCCTTT	(TTA)₆	283	Pc = 2 Pt = 2	Pc = 1.01 Pt = 1.77	Pc = 0.01 Pt = 0.64	Pc = 0.01 Pt = 0.43	Pc = -0.005 Pt = -0.471	Pc = 1 Pt = 2	Pc = 1 Pt = --
CAU3	F: TTCTAACCTAAGGCGCTTGC R: TGGCAAAAGTCAACAGACCA	(AAG)₆	224	Pc = 3 Pt = 5	Pc = 1.02 Pt = 2.79	Pc = 0.01 Pt = 0.97	Pc = 0.02 Pt = 0.64	Pc = 0.496 Pt = -0.509	Pc = 1 Pt = 3	Pc = 2 Pt = 2
CAU6	F: GCAACTTGTCCCTGTGATGA R: TTTCATCCATGCACTCCAAA	(AT)₇	302	Pc = 3 Pt = 5	Pc = 2.02 Pt = 3.83	Pc = 1.00 Pt = 0.97	Pc = 0.50 Pt = 0.74	Pc = -0.981 Pt = -0.311	Pc = 2 Pt = 4	Pc = 1 Pt = 1
CAU7	F: GGAAGGAACCACAGGATCAA R: AGGCTTGGTGTTTCATGAGG	(AAT)₆	276	Pc = 3 Pt = 9	Pc = 2.02 Pt = 4.11	Pc = 1.00 Pt = 1.00	Pc = 0.50 Pt = 0.76	Pc = -0.981 Pt = -0.322	Pc = 2 Pt = 4	Pc = 1 Pt = 5
CAU8	F: CCGAGAATCCCATCTTCTCA R: GCCATCTCCGTTTTATCCAA	(AG)₆	355	Pc = 1 Pt = 5	Pc = 1.00 Pt = 2.68	Pc = 0.00 Pt = 1.00	Pc = 0.00 Pt = 0.63	Pc = N/A Pt = -0.594	Pc = 1 Pt = 3	Pc = -- Pt = 2
CAU11	F: AATGGATCGCTGTCTCCATC R: CGGTCCCACATCATTCTCTT	(CT)₁₉	347	Pc = 4 Pt = 7	Pc = 2.10 Pt = 3.99	Pc = 1.00 Pt = 0.98	Pc = 0.52 Pt = 0.75	Pc = -0.909 Pt = -0.313	Pc = 2 Pt = 4	Pc = 2 Pt = 3
CAU12	F: ACCATTCCATCCTCTCCAAG R: GAATGGGCGGATCTAGGTTT	(TC)₁₉	217	Pc = 3 Pt = 3	Pc = 1.17 Pt = 1.85	Pc = 0.16 Pt = 0.67	Pc = 0.15 Pt = 0.46	Pc = -0.081 Pt = -0.458	Pc = 1 Pt = 2	Pc = 2 Pt = 1
CAU14	F: CTTTGCGTCTGACCTTGAAA R: TGTCCGGCTAGCTATTTTGG	(TC)₁₅	362	Pc = 3 Pt = 4	Pc = 2.02 Pt = 2.19	Pc = 1.00 Pt = 0.33	Pc = 0.50 Pt = 0.54	Pc = -0.981 Pt = 0.397	Pc = 2 Pt = 3	Pc = 1 Pt = 1
TRU1	F: CCATTGTTGTTTAGCCTGGAA R: ATCTCAGCTCAAGCCTGTGG	(TG)₈	254	Pc = 1 Pt = 5	Pc = 1.00 Pt = 2.01	Pc = 0.00 Pt = 0.34	Pc = 0.00 Pt = 0.50	Pc = N/A Pt = 0.315	Pc = 1 Pt = 3	Pc = -- Pt = 2
TRU2	F: CGAAACGTAAAACGCTCGAA R: ATTGCAACAGGACCTCCAAA	(TC)₁₃	258	Pc = 3 Pt = 4	Pc = 2.02 Pt = 1.55	Pc = 1.00 Pt = 0.34	Pc = 0.50 Pt = 0.36	Pc = -0981 Pt = 0.036	Pc = 2 Pt = 2	Pc = 1 Pt = 2
TRU5	F: TGTTACATGCCGTGGGACTA R: TTTGGGGTAATCGATGAAGG	(CT)₁₆	344	Pc = 2 Pt = 4	Pc = 1.01 Pt = 1.44	Pc = 0.01 Pt = 0.33	Pc = 0.01 Pt = 0.31	Pc = -0.005 Pt = -0.077	Pc = 1 Pt = 2	Pc = 1 Pt = 2
TRU7	F: TATGCAAGTCTGCCGTTGAG R: CTTTCTGGCGAGTTGAGAGG	(TC)₃₆	305	Pc = 3 Pt = 3	Pc = 2.02 Pt = 2.99	Pc = 1.00 Pt = 0.63	Pc = 0.50 Pt = 0.66	Pc = -0.981 Pt = 0.061	Pc = 2 Pt = 3	Pc = 1 Pt = --
TRU9	F: CCAAGGGTGCAATGTTGTAA R: GGTTCCACCAGAACAAAGGA	(CT)₁₀	400	Pc = 3 Pt = 6	Pc = 2.02 Pt = 2.23	Pc = 1.00 Pt = 0.37	Pc = 0.50 Pt = 0.55	Pc = -0.981 Pt = 0.320	Pc = 2 Pt = 3	Pc = 1 Pt = 3
TRU14	F: TGTACGTTGAGGATTGACTCG R: CTGGTCGTTCACATCATTGG	(CT)₇	261	Pc = 4 Pt = 3	Pc = 2.04 Pt = 2.16	Pc = 1.00 Pt = 0.31	Pc = 0.51 Pt = 0.54	Pc = -0.962 Pt = 0.418	Pc = 2 Pt = 3	Pc = 2 Pt = --
TRU15	F: CCCATGAGGGGAGTGATCTA R: GATCAACCTGACCCCAAGAA	(AG)₈	328	Pc = 1 Pt = 7	Pc = 1.00 Pt = 4.03	Pc = 0.00 Pt = 1.00	Pc = 0.00 Pt = 0.75	Pc = N/A Pt = -0.330	Pc = 1 Pt = 4	Pc = -- Pt = 3

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[tfnI] $: Pc = Plinia cauliflora ; Pt = Plinia trunciflora ; N/A = not applied.