Bidens mosaic virus: detection by RT-PCR and identification of Galinsoga parviflora as a new natural host of the virus

Abstract

Bidens mosaic virus (BiMV) is a tentative species of the genus Potyvirus and infects lettuce (Lactuca sativa). In the absence of efficient methods for the diagnosis of the virus, the objective of this work was to develop specifics primers for a one-step RT-PCR test using total RNA. The primer pairs 8851sens (5'AGG CAG TTC GCA CGG CAT AC 3´) and 9211ant (5´ CTT CAT CTG GAT GTG TGC TTC 3´) were considered very efficient for the detection of BiMV and the virus was found naturally infecting Galinsoga parviflora, a new host of BiMV, commonly found in commercial lettuce crops.

Potyvirus; lettuce; BiMV