Biochemical characterization of sugarcane (Saccharum spp.) cultivars: isoenzymes, solubre protein and brix value

Abstract

In the present work the isoenzyme profiles of the enzymes esterase and peroxidase, the level of total soluble protein and the soluble solids (sucrose) were determined of the following sugarcane (Saccharum spp.) cultivare: NA 56-79; IAC 52-150; IAC 64-257; SP 70-1143; SP 71-3146; SP 71-3149; SP 71-1406; SP 71-6163; SP 71-61-68 and SP 71-799. Esterase isoenzymes showed a specific electrophoretic pattern for each one of the cultivare, while the peroxidase allowed to arrange the cultivare in groups, each one with a specific electrophoretic pattern. The isoenzymes of both esterase and peroxidase were constant in a given cultivar. Total soluble protein levels and soluble solids (sucrose, Brix value) varied among the cultivare. Statistical analysis showed that these biochemical parameters are useful for the characterization of the cultivare under study.

chemotaxonomy; sugarcane; isoenzymes; esterase; peroxidase