Rendimento dos meios seletivos para bactérias do gênero FusobacteriumAbstract
Gingival debris was collected with sterile periodontal scalers from patients with healthy gingiva and from periodontally involved patients to compare the effectiveness of selective media for isolation of Fusobacterium. The material was weighed out on aluminum foil (previously weighed), homogenized, diluted into phosphate buffer (0,067 M-pH1,2), and 0,1 ml was plated out on blood agar, blood ugar-vancomycin (7,5 ug/ml), Omata & Disraely agar, Omata & Disraely agar without horse serum, McCarthy & Disraely agar, Onisi agar with horse serum, and Onisi agar with pH 7,2. In all cases the plates were incubated at 37°C, in anaerobic Brewer jars with 95% nitrogen and 5% carbon dioxide for fodr days. McCarthy & Snyder agar was so effective as the plain blood agar in relation to the isolation of Fusobacterium; on the other hand, it inhibited the concomitant flora. Omata & Disraely agar (with crystal violet and streptomycin) was inhibitory for fusobacteria, too. A few number of fusobacteria was isolated in the Onisi medium (without serum) . Probably those strains did not require natural proteins.