Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Lima, Giselle Jucá de; Scavuzzi, Alexsandra Maria Lima; Beltrão, Elizabeth Maria Bispo; Firmo, Elza Ferreira; Oliveira, Érica Maria de; Oliveira, Sibele Ribeiro de; Rezende, Antônio Mauro; Lopes, Ana Catarina de Souza

doi:10.1590/0037-8682-0526-2019

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Short Communication • Rev. Soc. Bras. Med. Trop. 53 • 2020 • https://doi.org/10.1590/0037-8682-0526-2019 linkcopy

Identification of plasmid IncQ1 and NTE_KPC-IId harboring bla _KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION: This study investigated the genetic environment of bla _KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates.

METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing.

RESULTS: The bla _KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTE_KPC-IId). It was detected on a plasmid belonging to the IncQ1 group.

CONCLUSIONS To our knowledge, this is the first report of the presence of the bla _KPC-2 gene in the NTE_KPC-IId element carried by plasmid IncQ1 from infections in Brazil.

Keywords:
Klebsiella pnemoniae ; IncQ1; bla _KPC-2 ; NTE_KPC-IId

Isolates	Origin	ERIC-PCR profile	Resistance profile
K3R2	Peritoneal fluid	P1	AMP, APS, CFL, CFZ, CFP, CXM, CAZ, CRO, CFX, CIP, ERT, IPM, MER
K4R2	Blood culture	P2	AMP, APS, CFL, CFZ, CFP, CXM, CAZ, CRO, CFX, CIP, ERT, IPM, MER
K6R2	Cerebrospinal fluid	P3	AMP, APS, CFL, CFZ, CFP, CXM, CAZ, CRO, CFX, CIP, ERT, IPM, MER
K1E	Blood culture	P5	AMP, APS, CFL, CFZ, CFP, CAZ, CRO, CFX, CIP, ERT, GEN, IPM, MER

Isolates	K3R2	K4R2	K6R2	K1E	IncQ (M28829.1)
Reference	This study	This study	This study	This study	Scholz et al. (1989)
Size of the sequenced plasmid DNA	27,508 bp	27,325 bp	59,776 bp	84,800 bp	8,684 bp
GC	57.95%	58.13%	55.35%	53.84%	61%
CDS	31	31	70	94	40
Inc	IncQ1 and NTP	IncQ1 and NTP	IncQ1 and NTP	IncQ1 and NTP	IncQ1 and NTP
Resistance genes	bla _KPC-2	bla _KPC-2	bla _KPC-2	bla _KPC-2	str sul
Mobility and replication	mobA	mobA	mobA	mobA	mobA
Proteins	mobB	mobB	mobB	mobB	mobB
	mobC	mobC	mobC	mobC	mobC
	repA	repA	repA	repA	repA
	repB	repB	repB	repB	repB
	repC	repC	repC	repC	repC
	repF	repF	repF	repF	repF

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E-mail: rsbmt@sbmt.org.br

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[TFN1] K:Klebsiella pneumoniae; R2: public hospital; E: private hospital; P: profile; AMP: ampicillin; APS: ampicillin/sulbactam; CFL: cefalotin; CFZ: cefazolin; CFP: cefepime; CFX: cefoxitin, CAZ: ceftazidime; CRO: ceftriaxone; CXM: cefuroxime; CIP: ciprofloxacin; ERT: ertapenem; GEN: gentamycin, IPM: imipenem; MER: meropenem.