In vitro Regeneration of the 'Piel de Sapo' and 'Cantaloupe' Melon

Freire, Francisco B. S.; Oliveira, Frederico I. C. de; Carvalho, Alexya V. F.; Campos, Arlene S.; Carvalho, Ana C. P. P. de; Aragão, Fernando A. S. de

doi:10.1590/1983-21252025v3812175rc

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Scientific Article • Rev. Caatinga 38 • 2025 • https://doi.org/10.1590/1983-21252025v3812175rc linkcopy

In vitro Regeneration of the 'Piel de Sapo' and 'Cantaloupe' Melon

Regeneração in vitro de meloeiro ‘Pele de Sapo’ e ‘Cantaloupe’

Authorship SCIMAGO INSTITUTIONS RANKINGS

Melon cultivation is of significant socioeconomic importance, especially in the northeast of Brazil. In breeding programs that employ biotechnological techniques, developing efficient methods of in vitro plant regeneration is essential for this species. The aim of this study was to develop a regeneration protocol using cotyledonary explants. The experimental design was completely randomised in a 2 x 5 factorial scheme, with two melon cultivars ('Piel de Sapo' and 'Cantaloupe') x five concentrations of 6- benzylaminopurine (BAP) (0, 4.44, 8.88, 13.32, and 17.75 μM), with five replications of five test tubes, each containing one explant. The percentage of cotyledons that formed calluses and buds, the number of buds formed per callus, and the fresh weight of the callus were evaluated 35 days after the in vitro inoculation of the explants. Data were submitted to analysis of variance, with the mean values compared between cultivars using Tukey’s test, and regression analysis between the BAP doses. No buds were formed in the culture medium containing no BAP, so it was necessary to add BAP to both cultivars. In the culture medium containing 8.88 μM BAP, 68% of the calluses in 'Cantaloupe' formed buds, while for 'Piel de Sapo', buds were formed by 12% of the calluses only. The 'Cantaloupe' cultivar recorded the highest values for fresh callus weight and number of regenerated buds per explant, indicating a better in vitro response than that of 'Piel de Sapo'.

Keywords:
Cucumis melo L; Organogenesis; Plant tissue culture.

SV	DF	Mean Square
SV	DF	EC	CB	NBC	FWC
Cultivar (C)	1	2888.0000 *	16200.0000 *	30.3888 *	17.8802 *
BAP (B)	4	3 528.0000 *	4560.0000 *	8.8219*	11.5267 *
CxB	4	2888.0000 *	1800.0000 *	4.1374*	1.6329 *
Breakdown CxB
‘Cantaloupe’	4	16.0000 ^ns	5960.0000 *	10.5593 *	10.6538 *
‘Piel de Sapo’	4	6400.0000 *	400.0000 ^ns	2.4000 ^ns	2.5059 *
Residual	40	28.0000	224.0000	1.2674	0.0409
Corrected total	49	-	-	-	-
CV (%)	-	5.78	49.89	67.03	10.89

				BAP Concentration (μM)
Variable	Melon Cultivar	0	4.44	8.88 13.32	17.75	Mean
EC (%)	‘Piel de Sapo’	20.00 b	100.00 a	100.00 a 100.00 a	100.00 a	84.00 b
	‘Cantaloupe’	96.00 a	100.00 a	100.00 a 100.00 a	100.00 a	99.20 a
Mean		58.00	100.00	100.00 100.00	100.00	91.60
CB (%)	‘Piel de Sapo’	0.00 a	12.00 a	12.00 b 28.00 b	8.00 b	12.00 b
	‘Cantaloupe’	0.00 a	28.00 a	68.00 a 88.00 a	56.00 a	48.00 a
Mean		0.00	20.00	40.00 58.00	32.00	30.00
NBC	‘Piel de Sapo’	0.00 a	1.60 a	0.40 b 1.50 a	1.00 b	0.90 b
	‘Cantaloupe’	0.00 a	2.67 a	3.78 a 2.63 a	3.22 a	2.46 a
Mean		0.00	2.13	2.09 2.07	2.11	1.68
FWC (g)	‘Piel de Sapo’	0.07 a	1.18 b	1.50 b 1.76 b	1.78 b	1.26 b
	‘Cantaloupe’	0.25 a	1.67 a	3.40 a 3.65 a	3.31 a	2.46 a
Mean		0.16	1.43	2.45 2.71	2.54	1.86

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[TFN1] ^*
= significant by analysis of variance at 5% probability; ns = not significant by analysis of variance at 5% probability.