Genetic charactezation of Calophyllum brasiliense Camb. in two populations of riparian forest

Abstract

Calophyllum brasiliense Camb. is a predominant species in areas with high water saturation such as the riparian forests in southern Minas Gerais State. The objective of this research was to study the genetic variability of C. brasiliense populations. Twenty individuals were sampled from two populations located in Funil Dam in Lavras-MG. Isozyme eletrophoresis analysis provided evidence of 17 alleles distributed in 8 loci, which were represented in five enzymatic systems: alpha-esterase, beta esterase, acid phosphatase, malate dehydrogenase and transaminase oxalacetatum glutamate. The diversity indexes showed a low number of alleles per loci in both populations, pop I (1.75) and pop II (1.50). The polymorphic loci percentage (P) were 37.5% and 50% in populations I and II respectively. The mean heterozygosities were 0.119 and 0.111, while the expected was 0.131 and 0.112. The number of migrants (Nm) between populations was 2.70. The estimated effective size was 18 individuals for population I, and 19 for population II.

Effective size; genetic variability; isozymes