Improved model for the establishment and evaluation of detrusor overactivity in female Wistar rats

Zhang, Nian-zhao; Ma, Lin; Zhang, Jian-bo; Chen, Jun

doi:10.1590/S1677-5538.IBJU.2014.03.17

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REVIEW ARTICLE • Int. braz j urol 40 (3) • may-jun 2014 • https://doi.org/10.1590/S1677-5538.IBJU.2014.03.17 linkcopy

Improved model for the establishment and evaluation of detrusor overactivity in female Wistar rats

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Objective To improve the model for establishment and evaluation of detrusor overactivity in female Wistar rats.

Materials and Methods We ligated the perineal urethra of female Wistar rats and then performed filling cystometry. The probability of detrusor overactivity, bladder capacity, peak voiding pressure and histological changes were investigated.

Results Detrusor overactivity ratio of the obstruction group was 32.4%. Bladder capacity increased from 0.273 ± 0.036mL in control group to 0.89 ± 0.19mL in detrusor overactivity group (P < 0.001), and peak voiding pressure increased from 45.9 ± 4.1 cm.H₂O to 63.5 ± 17.4cm.H₂O (P = 0.007). For obstruction group, compared to no detrusor overactivity rats, detrusor overactivity rats had higher bladder capacity (0.89 ± 0.19mL versus 0.43 ± 0.09mL, P < 0.001) and higher peak voiding pressure (63.5 ± 17.4cm.H₂O versus 44.8 ± 6.2cm.H₂O, P = 0.005). Detrusor overactivity rats were classified according to peak voiding pressure (49.2 ± 4.2cm.H₂O versus 80.8 ± 7.1cm.H₂O, P < 0.001). Moreover, bladder weight increased significantly in detrusor overactivity rats (P = 0.003, P = 0.028) and detrusor histological hypertrophy was observed.

Conclusions Ligating perineal urethra and filling cystometry with intra-urethral cannula approach is a simple and easily reproducible method to establish and evaluate the model of detrusor overactivity in rats.

Lower Urinary Tract Symptoms; Urinary Bladder; Urodynamics

Groups	n (%)	BC (mL)	P	VP (cm.H₂O)	P
ICI	50	0.18 ± 0.04		38.8 ± 3.2
CI	44
Control	10	0.273 ± 0.036	< 0.001^a	45.9 ± 4.1	0.007^a
Obstruction	34		< 0.001^b		0.005^b
Non-DO	23 (67.6)	0.43 ± 0.09		44.8 ± 6.2
DO	11 (32.4)	0.89 ± 0.19	0.716^c	63.5 ± 17.4	< 0.001^c
group 1	6 (54.5)	0.91 ± 0.21		49.2 ± 4.2
group 2	5 (45.5)	0.87 ± 0.19		80.8 ± 7.1

Groups	n (%)	BM(g)	P	BW(g)	P
Control	10	311 ± 8	0.244^a	0.127 ± 0.015	0.003^a
Obstruction	34		0.500^b		0.028^b
Non-DO	23 (67.6)	307 ± 13		0.159 ± 0.041
DO	11 (32.4)	302 ± 24	0.572^c	0.226 ± 0.085	0.111^c
group 1	6 (54.5)	306 ± 23		0.189 ± 0.068
group 2	5 (45.5)	297 ± 27		0.271 ± 0.087

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[TFN01t01] ^a: DO versus control; ^b: DO versus Non-DO; ^c: group 1 versus group 2

BC = Bladder capacity; VP = Peak voiding pressure; ICI = Initial cystometric investigation of the 50 female Wistar rats before the study; CI = Cystometric investigation 6 weeks later; DO = Detrusor overactivity