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Genetics and Molecular Biology
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Genetics of Microorganisms • Genet. Mol. Biol. 28 (1) • Mar 2005 • https://doi.org/10.1590/S1415-47572005000100025 linkcopy

Open-access A shortcut in phage screening technique

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Abstract

A simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.

phage screening; shortcut for Benton & Davis technique; single agar-plate

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