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Genetics and Molecular Biology
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Genetics of Microorganisms • Genet. Mol. Biol. 45 (2) • 2022 • https://doi.org/10.1590/1678-4685-GMB-2021-0289 linkcopy

Open-access Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

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Bacillus thuringiensis BR145 isolated from a soybean field in Southern Brazil showed toxicity against two important insect pests from soybean crop, Helicoverpa armigera, and Chrysodeixis includens, with LC50 0.294 µg.cm-2 and 0.277 µg.cm-2, respectively. We analyzed the genome of this strain through sequences obtained by Next Generation DNA Sequencing and de novo assembly. The analysis of the genome revealed insecticidal genes cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Ab, cyt1, and vip3Aa, suggesting the use of this strain in new strategies of biological control.

Keywords:
Bacillus thuringiensis ; insecticidal genes; virulence factors; Helicoverpa armigera ; Chrysodeixis includens

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