Purification, partial characterization and antimicrobial activity of Lectin from <i>Chenopodium Quinoa</i> seeds

POMPEU, Dávia Guimarães; MATTIOLI, Marcelo Augusto; RIBEIRO, Rosy Iara Maciel de Azambuja; GONÇALVES, Daniel Bonoto; MAGALHÃES, Juliana Teixeira de; MARANGONI, Sérgio; SILVA, José Antônio da; GRANJEIRO, Paulo Afonso

doi:10.1590/1678-457X.6823

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Research Paper • Food Sci. Technol (Campinas) 35 (4) • Oct-Dec 2015 • https://doi.org/10.1590/1678-457X.6823 linkcopy

Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds

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A novel lectin was isolated from the seeds of Chenopodium quinoa. To achieve this end, the crude extract from the quinoa was submitted to two purification steps, Sephadex G50 and Mono Q. The hemagglutinating activity showed that this lectin agglutinates human erythrocytes. Its activity is inhibited by glucose and mannose, and remained stable under a wide range of pH levels and temperatures. The quinoa lectin was found to be a heterodimeric lectin of approximately 60 kDa, consisting of two subunits of approximately 25 kDa and 35 kDa. This lectin had its antimicrobial activity tested against several bacteria strains and effectively inhibited three strains. These strains were all Gram-negative, making this lectin a promising antimicrobial tool.

Keywords:
antimicrobial; Chenopodium quinoa; glucose/mannose-specific; lectin; seeds

Step	Hemagglutinatting Activity (HU.100 μL^–1) *	Total Protein (mg)	Yield (%)
Crude Extract	64	28.8	100.0
Sephadex G50	32	8.99	31.2
MonoQ (GE)	16	0.836	9.3

Human Erythrocytes (ABO system)	Lower hemagglutinating concentration (μg/mL)
A	7.73
B	3.86
O	7.73
AB	15.46

Carbohydrates	MIC *
Agar-agar	NI**
Agarose	NI**
Corn starch	NI**
Celullose	NI**
Fructose	NI**
Fucose	NI**
Galactose	NI**
Glucose	3.25 mM
Glucosamine	NI**
Gum arabic	NI**
Lactose	NI**
Maltose	NI**
Mannitol	NI**
Mannose	3.25 mM
Methyl α- piranoside	NI**
Pectin	NI**
Raffinose	NI**
Ribose	NI**
Saccharose	NI**

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[TFN1] ^*
HU: Hemagglutinating Unit: the inverse of the hemagglutinating title, calculated as 2number of well presenting the highest dilution with visible hemagglutination.