Nutrition-enriched nanobubble oxygen and red-blue light spectrum enhanced plantlet growth and in vitro Dendrobium development

O oxigênio nanobolha enriquecido com nutrição e o espectro de luz vermelho-azul melhorou o crescimento e desenvolvimento de plântulas de Dendrobium in vitro

An effective micropropagation system for Dendrobium is required to support seedling production. The effects of light quality with nutrition-enriched nanobubble (NB) and oxygen on Dendrobium plantlet growth and development were evaluated. Plantlets were placed on a growth medium (enriched or without NB oxygen) with vermiculite-perlite (1:1) and irradiated by fluorescent, red-blue spectrum ratio of (3:1, 1:1, 1:3), and red. The NB and dissolved oxygen in liquid nutrition and red-blue spectrum enhanced Dendrobium’s morphological and physiological characteristics in vitro sugar-free. The TF-NB-1R1B increased fresh weight and plantlet growth rate, but TF-NB-1R3B increased pigments. The shift from heterotrophic to autotrophic micropropagation is indicated by stomatal closer and upregulated CAT, PEPCK, and RBC genes.

Key words:
Dendrobium; LED lighting; micropropagation; nanobubble oxygenation; photomorphogenesis