Karyotype and fluorescent banding in the interspecific F<sub>1</sub> hybrid <i>Passiflora</i> 'Lamepiana', confirmed by GISH

Santos, Aline Pinto dos; Souza, Margarete Magalhães; Silva, Gonçalo Santos; Moreira, Rita de Cássia Teixeira

doi:10.1590/1984-70332025v25n2a24

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ARTICLE • Crop Breed. Appl. Biotechnol. 25 (2) • 2025 • https://doi.org/10.1590/1984-70332025v25n2a24 linkcopy

Karyotype and fluorescent banding in the interspecific F₁ hybrid Passiflora 'Lamepiana', confirmed by GISH

Authorship SCIMAGO INSTITUTIONS RANKINGS

Passiflora 'Lamepiana' is the F₁ hybrid from the cross between P. vitifolia and P. hatschbachii with ornamental characteristics. Based on cytogenetics techniques, this study aimed to confirm interspecific hybridization and characterize the karyotype of the hybrids and their parents using cytogenetic techniques. For all genotypes the predominance of metacentric chromosomes with two satellite pairs and a diploids karyotypes (2n = 18) were observed. It was possible to determine the occurrence of two satellite pairs in both the parents of the hybrids. The use of simultaneous probes of the F1 parents prepared according to GISH technique allowed to identify two chromosome lots each one inherited from both parents, confirming the hybrid character of the plants. Besides confirming hybridization, GISH did not demonstrate the existence of visible chromosomal alterations but rather cytogenetic stability in the hybrids.

Keywords:
Passionflower; karyomorphology; CMA ₃ /DAPI banding; molecular cytogenetics

P. vitifolia	Characteristic
Chromosome pair	LA	SA	CLC	r	CT
1	2.40	1.73	4.12	1.39	M
2	2.13	1.53	3.65	1.39	M
3	1.93	1.35	3.28	1.42	M
4	1.78	1.27	3.05	1.40	M
5	1.67	1.19	2.86	1.40	M
6	1.56	1.14	2.69	1.37	M
7	1.50	1.07	2.57	1.40	M
8	1.35	1.00	2.35	1.35	M
9	1.19	0.90	2.08	1.33	M
Standard deviation	0.38	0.26	0.64
P. hatschbachii	Characteristic
Chromosome pair	LA	SA	CLC	r	CT
1	2.20	1.26	3.46	1.38	M
2	2.03	1.23	3.26	1.29	M
3	1.53	1.16	2.69	1.32	M
4	1.42	1.08	2.5	1.32	M
5	1.32	1.07	2.38	1.23	M
6	1.29	1.01	2.3	1.29	M
7	1.25	0.90	2.16	1.39	M
8	1.24	0.82	2.06	1.50	SM
9	1.02	0.76	1.78	1.33	M
Standard deviation	0.22	0.18	0.55
Means of hybrids HD 26(102, 104, 143 and 146)	Characteristic
Chromosome	LA	SA	CLC	r	CT
1	2.16	1.61	3.76	1.34	M
2	1.89	1.41	3.47	1.38	M
3	1.83	1.27	3.11	1.43	M
4	1.69	1.24	2.93	1.37	M
5	1.60	1.19	2.79	1.33	M
6	1.53	1.16	2.71	1.32	M
7	1.52	1.14	2.66	1.33	M
8	1.44	1.09	2.54	1.31	M
9	1.37	1.07	2.45	1.29	M
10	1.33	1.04	2.37	1.29	M
11	1.30	1.02	2.31	1.28	M
12	1.28	0.98	2.25	1.30	M
13	1.21	0.95	2.17	1.29	M
14	1.19	0.93	2.11	1.28	M
15	1.12	0.86	1.98	1.29	M
16	1.08	0.82	1.89	1.30	M
17	1.02	0.76	1.78	1.33	M
18	0.89	0.68	1.58	1.32	M
Standard deviation	0.33	0.23	0.57

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Crop Breeding and Applied Biotechnology Universidade Federal de Viçosa, Departamento de Fitotecnia, 36570-000 Viçosa - Minas Gerais/Brasil, Tel.: (55 31)3899-2611, Fax: (55 31)3899-2611 - Viçosa - MG - Brazil
E-mail: cbab@ufv.br

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[TFN1] (SA) Short Arm; (LA) Long Arm; (SAT) Satellite; (CLC) Total Chromosome Length; r (LA/SA) Long Arm to Short Arm Ratio; (CT) Chromosome Type; (M) Metacentric; (SM) Submetacentric.

Karyotype and fluorescent banding in the interspecific F1 hybrid Passiflora 'Lamepiana', confirmed by GISH

Karyotype and fluorescent banding in the interspecific F₁ hybrid Passiflora 'Lamepiana', confirmed by GISH