Adhesion, biofilm and genotypic characteristics of antimicrobial resistant <i>Escherichia coli</i> isolates

Cergole-Novella, Maria C.; Pignatari, Antonio C.C.; Guth, Beatriz E.C.

doi:10.1590/S1517-838246120140077

Medical Microbiology • Braz. J. Microbiol. 46 (1) • Jan-Mar 2015 • https://doi.org/10.1590/S1517-838246120140077 linkcopy

Adhesion, biofilm and genotypic characteristics of antimicrobial resistant Escherichia coli isolates

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Aggregative adherence to human epithelial cells, most to renal proximal tubular (HK-2) cells, and biofilm formation was identified among antimicrobial resistant Escherichia coli strains mainly isolated from bacteremia. The importance of these virulence properties contributing to host colonization and infection associated with multiresistant E. coli should not be neglected.

adherence; biofilm; extraintestinal Escherichia coli; antimicrobial resistance; virulence genes

E. coli a	Clinical diagnosis	Serotype	Antimicrobial resistanceb	bla	Virulence markersd	Expression of				Biofilm formation on glass	Adherence to cells f			Invasion Index (%)

						Type 1 fimbria	α-Hly	Curlie	Cellulose		CaCo-2	HeLa	HK-2g
34	Acute gastroenterocoliti	O102:H6	Amp, Ctx, Cip, Nal, Chlo, Str, Gen, Kan, Sut, Tet	TEM-1, CTX-M-14	crl, csgA, fimH, irp2, traT	−	−	−	−	+	+	+	+	1.5
35	Acute gastroenterocoliti	O102:H7	Amp, Ctx, Cip, Nal, Chlo, Str, Gen, Kan, Sut, Tet	TEM, CTX-M c	crl, csgA, fimH, irp2, traT	−	−	−	−	+	+	+	+	3.0
36	Pneumonia	ONT:HNM	Amp, Ctx, Cfz, Cef, Cip, Nal, Str, Kan, Sut, Tet	OXA-1, CTX-M-15	crl, csgA, fimH, irp2, traT	−	−	−	−	−	−	−	+	3.2
38	Septic shock	ONT:HNM	Str	-	crl, csgA, fimH, irp2, iutA1, spates, traT	+	−	−	−	−	−	−	−	1.4
39	Sepsis	ONT:H27	Str, Sut, Tet	-	crl, csgA, fimH	+	−	+	−	+	+	+	+	0.8
40	Urinary tract infection	O4:H31	Amp, Str, Sut, Tet	TEM	crl, csgA, cvaC, fimH, ibe10, irp2, iutA1, kpsMTII, spates, traT, vat	−	−	−	+	−	+	+	+	2.2
41	Urinary tract infection	O102:H30	Amp, Cip, Nal, Chlo, Str, Sut, Tet	-	crl, csgA, fimH, kpsMTII, traT	+	−	+	−	+	−	−	+	1.5
42	Urinary tract infection	O1:HNM	Str	-	crl, csgA, fimH, iha, irp2, iutA1, papC, spates, traT	−	−	−	−	−	+	−	−	1.0
43	Cholangitis	ONT:HNM	Tet	-	crl, csgA, fimH, kpsMTII	−	−	+	−	+	−	−	−	0
44	Urinary tract infection	ONT:H4	Amp, Cip, Nal, Str, Sut, Tet	TEM	crl, csgA, fimH, iha, irp2, iutA1, spates, traT	−	−	−	−	+	−	−	−	2.4
45	Urinary tract infection	ONT:H31	Str, Sut, Tet	-	crl, csgA, cvaC, fimH, ibe10, iutA1, irp2, kpsMTII, spates, traT, vat	+	−	−	−	+	+	+	+	0.6
47	Urinary tract infection	O6:H1	Amp, Str	-	crl, csgA, fimH, cnf1, iha, irp2, iutA1, papC, kpsMTII, spates, traT, vat	−	+	−	−	+	CD	+	CD	0.7

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Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

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[TFN01] ^a
E. coli isolates 34 (feces) and 35 (blood culture) were obtained at the same day from the same patient; E. coli 36 (tracheal secretion) and all the remaining isolates were obtained from blood cultures (Cergole-Novella et al., 2010).

[TFN02] ^b
Nalidixic acid (Nal), ampicillin (Amp), kanamicin (Kan), cefepime (Cef), cefotaxime (Ctx), ceftazidime (Cfz), ciprofloxacin (Cip), chloramphenicol (Chlo), streptomycin (Str), gentamicin (Gen), trimethoprim-sulfamethoxazole (Sut) and, tetracycline (Tet).

[TFN03] ^c
DNA sequencing was not done for β-lactamases gene (bla) TEM and CTX-M.

[TFN04] ^d
E. coli isolates were negative by PCR for aggR, aatA, aggA and aafA, espI, espP, afa, and sfa.

[TFN05] ^e
Isolate 43 presented both curli+ and curli− colonies.

[TFN06] ^f
All isolates that presented or not adherence to different epithelial cells were tested in 3 and 6 h assays, except for the isolates that presented adherence to HeLa cells that were positive in 3 h assays; CD, cell detachment.

[TFN07] ^g
All E. coli isolates presented aggregative adherence to HK-2 cells, except isolate 40 that presented a non-defined adherence pattern.