Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium

Santisi, Santina; Cappello, Simone; Catalfamo, Maurizio; Mancini, Giuseppe; Hassanshahian, Mehdi; Genovese, Lucrezia; Giuliano, Laura; Yakimov, Michail M.

doi:10.1590/S1517-838246120131276

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Environmental Microbiology • Braz. J. Microbiol. 46 (2) • Apr-Jun 2015 • https://doi.org/10.1590/S1517-838246120131276 linkcopy

Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

Alcanivorax ; Pseudomonas ; Rhodococcus ; bioremediation; bioaugmentation

Probe	Sequence (5′ to 3′) of probe	Specificity	Source
NON-Eub338	ACA TCC TAC GGG AGG C	Negative Control	(Wallner et al., 1993)
Eub338	GCT GCC TCC CGT AGG AGT	Domain Bacteria	(Amann et al., 1990)
Alk	CG ACG CGA GCT CAT CCA TCA	Alcanivorax genus	(Karner and Fuhrman, 1997)

n-alkanes	Natural sea water

	A	P	R	A+P	A+R	P+R	A+P+R
C₁₂	95	80	82	86	100	90	99
C₁₃	69	52	57	68	94	92	96
C₁₄	56	45	48	52	74	94	77
C₁₅	42	42	49	51	82	94	92
C₁₆	46	46	52	57	81	93	92
C₁₇	51	48	53	57	79	91	92
C₁₈	52	49	56	57	81	89	93
C₁₉	63	63	68	65	82	88	94
C₂₀	60	59	64	62	78	86	93
C₂₁	66	67	70	68	79	82	93
C₂₂	68	69	72	67	80	79	94
C₂₃	77	77	80	74	84	79	95
C₂₄	66	68	71	62	78	69	94
C₂₅	74	75	78	70	82	66	95
C₂₆	75	77	79	71	84	66	95
C₂₇	75	76	78	70	83	53	95
C₂₈	75	76	79	70	84	47	95
C₂₉	73	73	77	67	83	37	95
C₃₀	76	77	81	72	87	31	96

n-alkanes	Natural sea water + inorganic nutrient

	A	P	R	A+P	A+R	P+R	A+P+R

C₁₂	100	87	100	100	100	100	100
C₁₃	100	88	97	100	100	100	100
C₁₄	100	89	95	100	100	100	100
C₁₅	100	82	91	100	100	100	100
C₁₆	100	84	95	100	100	90	100
C₁₇	100	85	95	100	100	81	100
C₁₈	100	83	94	100	100	94	100
C₁₉	100	83	90	100	100	80	100
C₂₀	100	82	92	100	100	80	100
C₂₁	100	81	90	100	100	77	100
C₂₂	100	81	92	100	100	72	100
C₂₃	100	83	90	100	100	71	100
C₂₄	100	76	87	100	100	63	100
C₂₅	100	77	90	100	100	68	100
C₂₆	100	77	86	100	100	70	100
C₂₇	100	75	87	100	100	71	100
C₂₈	100	75	86	100	100	69	100
C₂₉	100	75	81	100	100	71	100
C₃₀	100	77	87	100	100	62	100

Code	Natural sea water	Natural sea water+IN
A	64	100
P	48	64
R	64	90
A+R	66	100
A+P	83	100
P+R	73	60
A+P+R	93	100

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Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

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