Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Leal, C.A.G.; Carvalho-Castro, G.A.; Cottorello, A.C.; Leite, R.C.; Figueiredo, H.C.P.

doi:10.1590/S1517-83822013005000054

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Veterinary Microbiology • Braz. J. Microbiol. 44 (3) • Sept 2013 • https://doi.org/10.1590/S1517-83822013005000054 linkcopy

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

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Abstract

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

clinical samples; sensitivity; real-time PCR; WSSV; WSD

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Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

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