Cytotoxicity and bioactivity of CIMMO DTA restorative cement in human activated-dental pulp cells in vitro

Bioceramic materials have been introduced in dentistry due to their physical, chemical, and biological properties, which are considered advanced in clinical practice. According to the manufacturer, CIMMO DTA bioceramic restorative cement is a biological bioceramic cement and was introduced recently at a low cost compared to other bioceramic materials. However, no studies evaluated the effect of CIMMO DTA on human dental pulp cells (HDPC) activated with lipopolysaccharide (LPS). Therefore, this study aimed to evaluate the cytotoxicity and the osteo/odontogenic differentiation potential in HDPC activated with LPS exposed to CIMMO DTA compared to Hydro-C. HDPC activated or not with LPS were cultured, plated, and exposed to different dilutions of CIMMO DTA and Hydro-C extracts. At 24h, 48h, and 72h, the cells were subjected to the MTT assay. The osteo/odontogenic mineralization potential of cells was evaluated through the Alizarin red S staining assay at a 21-day time point. Statistical analysis was performed using a two-way analysis of variance (ANOVA) followed by Tukey's post-test (p<0.05). In general, the tested materials unaffected the metabolic activity and viability of cells in all dilutions evaluated. The CIMMO DTA reduced the osteo/odontogenic differentiation potential of the HDPC according to qualitative and quantitative results at dilutions of 1:8. Therefore, the results of the present study suggested that CIMMO DTA presents limited bioactive potential on HDPC in vitro.

Key Words:
Bioceramic Restorative Cement; Cytotoxicity; Differentiation Potential Human Dental Pulp Cells.