Optimization of Parameters for Biosynthesis of Silver Nanoparticles Using Leaf Extract of <b>Aegle marmelos</b>

Christopher, JohnSamuel Godwin; Saswati, Banerjee; Ezilrani, PanneerSelvam

doi:10.1590/S1516-89132015050106

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Agriculture, Agribusiness and Biotechnology • Braz. arch. biol. technol. 58 (5) • Sep-Oct 2015 • https://doi.org/10.1590/S1516-89132015050106 linkcopy

Optimization of Parameters for Biosynthesis of Silver Nanoparticles Using Leaf Extract of Aegle marmelos

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The aim of this study was to optimize the biosynthesis of silver nanoparticles using leaves of Aegle marmelos as the primary source. The optimal reaction medium comprised 2:1 concentration of leaf extract and 6mM concentration of silver nitrate solution (pH 7. The biosynthesized silver nanoparticles were confirmed by UV-Vis spectroscopy at 420 nm, XRD and FTIR analysis. The antimicrobial properties of silver nanoparticles were confirmed with Bacillus subtilis andPseudomonas aeruginosa.

Key words:
AgNO₃ ; Aegle marmelos leaf; UV-Vis spectroscopy; XRD; FTIR

Concentration	Zone of inhibition (mm)
Concentration	Bacillus	Pseudomonas
60µL	4	8
80µL	9	11
100µL	13.5	14

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