Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species (<i>Capra falconeri heptneri</i>)

Locatelli, Yann; Vion, Colin; Duffard, Nicolas; Bon, Nicolas; Bernard, Jérémy; Binaud, Charly; Trontti, Nina; Baril, Gérard; Mermillod, Pascal

doi:10.1590/1984-3143-AR2024-0071

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THEMATIC SECTION: X INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION (ISABR) • Anim. Reprod. 21 (3) • 2024 • https://doi.org/10.1590/1984-3143-AR2024-0071 linkcopy

Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species (Capra falconeri heptneri)

Authorship SCIMAGO INSTITUTIONS RANKINGS

The objective of the present study was to transpose sperm freezing methodology from domestic goat to the Tadjik markhor (Capra falconeri heptneri) and to address the feasibility to develop IVP and artificial insemination using such frozen semen. Semen of different adult markhor males were successfully recovered by electro-ejaculation and were then frozen using caprine methodology. Frozen semen showed good survival rates at thawing and good fertility rates were assessed in heterologous in vitro fertilization system with goat oocytes. LOPU/IVF was applied for Tadjik markhor females allowing the first successful blastocyst production in vitro. In an applied program, we also transposed successfully intrauterine AI method with frozen/thawed semen to the Tadjik markhor.

Keywords:
semen; artificial insemination; embryo production; LOPU

Male samples	oocytes (n)	cleavage	cleavage
Male samples	oocytes (n)	24 hpi (%)	48 hpi (%)
Control	135	38.5 ^a	82.2^a
#1	120	0.8 ^b	10^b
#2	154	13^c	77.9^a
#3	159	8.8^c	76.1^a
#4	153	5.2 ^d	74.5^a

Control	192	14 ^a	84 ^a
#4	179	4.6b	80.4^a
#5	191	2.1 ^b	18.8^b
#6	181	9.3a	71.8^a

n LOPU sessions (total markhor females involved)	mean number of follicle aspirated	mean number of oocytes recovered
n LOPU sessions (total markhor females involved)	per female per LOPU session
9 (n=7)	13.63±1.53	8±0.74

Replicates	Oocytes n	Cleaved 48 hpi %	Morula D8pi (% cleaved )	Blastocyst D8pi (% from cleaved )	Total Morula + blastocyst D8pi (% from cleaved)
7	107	54.2%	12.1%	19.0%	31.0%

Year / month	AI time/ eCG	Female number	Semen /quality	pregnant
2017/12	52h/100 IU	3	Helsinki 1/ very poor	0/3
2018/12	52h/100 IU	3	Helsinki 2/ poor	0/3
2020/01	52h/100 IU	3	Helsinki 2#/good	0/3
2020/12	52h/100 IU	3	Helsinki 2#/good	0/3
2021/01	65h/100 IU	3	Helsinki 2#/good	1/3
2021/12	65h/100IU	3	Helsinki 2#/good	1/3

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Colégio Brasileiro de Reprodução Animal Coronel José dias Bicalho, 1224, CEP: , 31275-050, Belo Horizonte, MG - Brasil, Tel.: 55-31-3491 7122 - Belo Horizonte - MG - Brazil
E-mail: animreprod.journal@gmail.com

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[1] Within a column, values with different letters differ significantly (P < 0.05).