Approaches to improve <i>in vitro</i> survival, growth, and maturation of caprine oocytes: main results from LAMOFOPA-Brazil

Figueiredo, José Ricardo de; Silva, Ana Flávia Bezerra da; Lima, Laritza Ferreira de

doi:10.1590/1984-3143-AR2024-0059

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Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR) • Anim. Reprod. 21 (3) • 2024 • https://doi.org/10.1590/1984-3143-AR2024-0059 linkcopy

Approaches to improve in vitro survival, growth, and maturation of caprine oocytes: main results from LAMOFOPA-Brazil

Authorship SCIMAGO INSTITUTIONS RANKINGS

This brief review delves into the topic of in vitro follicle culture for in vitro embryo production, with a particular emphasis on goat models. Specifically, we examine the main findings from LAMOFOPA-Brazil over the last 20 years, highlighting the challenges posed by oxidative stress and epigenetic changes. Our focus is on strategies to improve follicular development and oocyte maturation. Furthermore, we underscore the valuable role of the antioxidant anethole in optimizing the efficacy of in vitro follicle culture and improving outcomes in in vitro embryo production.

Keywords:
anethole; oocyte growth; in vitro follicle culture; caprine

Parameters	*Treatments (best concentrations) vs.* cultured control**
↑ Survival	ANGII (10 ng/mL), BMP-15 (100 ng/mL), EGF (1 ng/mL), FGF-10 (50 ng/mL), GDF-9 (200 ng mL^-1), IGF-1 (50 ng/mL), KL (50 ng/mL), LIF (50 ng/mL), PDGF-BB (25 ng/mL), VEGF (200 ng mL^-1), GH (10 ng/mL), INS (10 ng/mL), VIP (10 ng/mL), S1P (1 ng/mL), AN (2000 µg/mL), AA (50 µg/mL), EUG (40 µM), JUS (0.3 mg/mL).
↑ Activation	BMP-15 (100 ng/mL), EGF (1 ng/mL), FGF-2 (50 ng/mL), GDF-9 (200 ng mL^-1), IGF-1 (50 ng/mL), KL (50 ng/mL), LIF (50 ng/mL), E2 (10 pg/mL), GH (10 ng/mL), IAA (40 ng/mL), INS (10 ng/mL), LH (100 ng/mL), S1P (1 ng/mL), AN (30 µg/mL), EUG (40 µM).
↑ Follicular diameter	BMP-15 (100 ng/mL), FGF-2 (50 ng/mL), FGF-10 (50 ng/mL), IGF-1 (50 ng/mL), KL (50 ng/mL), PDGF-BB (25 ng/mL), TGF-β (10 ng/mL), VEGF (10 ng mL^-1), ACT-A (100 ng/mL), FSH (50 ng/mL), E2 (10 pg/mL), GH (10 ng/mL), IAA (10 ng/mL), INS (10 ng/mL), LH (1 ng/mL), P4 (1 ng/mL), VIP (10 ng/mL), AN (30 µg/mL), AA (50 µg/mL), EUG (40 µM), MEL (1000 pM).
↑ Oocyte diameter	BMP-15 (100 ng/mL), GDF-9 (200 ng mL^-1), IGF-1 (50 ng/mL), NGF (1 ng/mL), PDGF-BB (25 ng/mL), VEGF (10 ng mL^-1), ACT-A (100 ng/mL), GH (10 ng/mL), IAA (10 ng/mL), VIP (10 ng/mL), AN (30 µg/mL), AA (50 µg/mL), EUG (40 µM), MEL (1000 pM).
↑ Antioxidant capacity or ↓ ROS	AN (30 µg/mL), EUG (40 µM).

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[1] Growth factors – ANGII: Angiotensin II; BMP-15: Bone morphogenetic protein 15; EGF: Epidermal growth factor; FGF-2: Fibroblast growth factor 2; FGF-10: Fibroblast growth factor 10; GDF-9: Growth differentiation factor 9; IGF-1: Insulin-like growth factor 1; KL: Kit ligand; LIF: Leukemia inhibitory factor; PDGF-BB: Platelet-derived growth factor-BB; TGF-β: Transforming growth factor beta; VEGF: Vascular endothelial growth factor. Hormones – ACT-A: Activin-A; E2: Estradiol; FSH: Follicle-stimulating hormone; GH: Growth hormone; IAA: Indole-3-acetic acid; INS: Insulin; LH: Luteinizing hormone; P4: Progesterone; VIP: Vasoactive intestinal peptide. Lipid – S1P: Sphingosine-1-phosphate. Antioxidants – AN: Anethole; AA: Ascorbic acid; EUG: Eugenol; JUS: Justicia insularis; MEL: Melatonin. ↑: Increased; ↓: Decreased; ROS: Reactive oxygen species.