Effect of taxifolin on oxidative gastric injury induced by celiac artery ligation in rats

Eken, Hüseyin; Cimen, Orhan; Cimen, Ferda Keskin; Kurnaz, Eray; Yildirim, Murat; Tasova, Volkan; Kurt, Nezahat; Pehlivanoglu, Kamil; Onk, Didem; Bilgin, Asli Ozbek

doi:10.1590/s0102-865020190040000004

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ORIGINAL ARTICLE, Experimental Surgery • Acta Cir. Bras. 34 (04) • 2019 • https://doi.org/10.1590/s0102-865020190040000004 linkcopy

Effect of taxifolin on oxidative gastric injury induced by celiac artery ligation in rats¹

Authorship SCIMAGO INSTITUTIONS RANKINGS

Purpose: To examine the effect of taxifolin on I/R induced gastric injury in rats using biochemical and histopatholohical methods.

Methods: Eighteen albino Wistar male rats equally grouped as; gastric I/R (I/R), 50 mg/kg taxifolin + gastric I/R (TAX+ I/R) and sham operation applied (SHAM). Ischemia induced for 1 hour, and reperfusion induced for 3 hours.

Results: Oxidant parameters like, Malondialdehyde (MDA) and Hydroxyguanine (8-OHdG) were higher, whereas total glutathione (tGSH) was lower in the I/R group according to SHAM group, histopathological findings such as marked destruction, edema, and proliferated dilated congested blood vessels were observed severely in the I/R group, whereas there was not any pathological finding except mild dilated congested blood vessels in the TAX+ I/R group.

Conclusion: The taxifolin can be clinically beneficial in the treatment of gastric injury due to I/R procedure.

Key words:
Taxifolin; Celiac Artery; Ischemia; Reperfusion; Rats

Authorship

person Hüseyin Eken

schoolAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Scientific, intellectual, conception and design of the study; manuscript preparation.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Scientific, intellectual, conception and design of the study; manuscript preparation.

0000-0001-6095-6002

person Orhan Cimen

schoolAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Conception and design of the study, manuscript preparation.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Conception and design of the study, manuscript preparation.

0000-0003-4723-5838

person Ferda Keskin Cimen

schoolAssistant Professor, Department of Pathology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Histopathological examinations, manuscript writing.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of Pathology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Histopathological examinations, manuscript writing.

0000-0002-1844-0827

person Eray Kurnaz

schoolMD, Department of General Surgery, Mengücek Gazi Training and Research Hospital, Erzincan, Turkey. Technical procedures, manuscript preparation. Mengücek Gazi Training and Research HospitalTurkeyErzincan, TurkeyMD, Department of General Surgery, Mengücek Gazi Training and Research Hospital, Erzincan, Turkey. Technical procedures, manuscript preparation.

0000-0002-9043-558X

person Murat Yildirim

schoolMD, Department of General Surgery, Zile State Hospital, Tokat, Turkey. Technical procedures, manuscript preparation. Zile State HospitalTurkeyTokat, TurkeyMD, Department of General Surgery, Zile State Hospital, Tokat, Turkey. Technical procedures, manuscript preparation.

0000-0002-4938-5103

person Volkan Tasova

schoolMD, Department of General Surgery, Sabuncuoglu Serafettin Training and Research Hospital, Amasya University, Turkey. Technical procedures, manuscript preparation.Amasya UniversityTurkeyTurkeyMD, Department of General Surgery, Sabuncuoglu Serafettin Training and Research Hospital, Amasya University, Turkey. Technical procedures, manuscript preparation.

0000-0003-3368-4421

person Nezahat Kurt

schoolPhD, Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey. Acquisition, analysis and interpretation of data; technical procedures.Ataturk UniversityTurkeyErzurum, TurkeyPhD, Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey. Acquisition, analysis and interpretation of data; technical procedures.

0000-0002-1685-5332

person Kamil Pehlivanoglu

schoolAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Manuscript preparation. Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Manuscript preparation.

0000-0003-2981-3964

person Didem Onk

schoolAssistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Technical procedures, critical revision.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Technical procedures, critical revision.

0000-0001-8849-0531

person Asli Ozbek Bilgin

schoolAssistant Professor, Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Statistics analysis, manuscript writing, critical revision, final approval.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Statistics analysis, manuscript writing, critical revision, final approval.

0000-0001-9027-4299

personCorrespondence: Prof. Asli Ozbek Bilgin Department of Pharmacology, Faculty of Medicine Erzincan Binali Yildirim University Turkey Phone: +904462261818
asliozbekbilgin@gmail.com

Conflict of interest:

none

SCIMAGO INSTITUTIONS RANKINGS

Assistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Scientific, intellectual, conception and design of the study; manuscript preparation.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Scientific, intellectual, conception and design of the study; manuscript preparation.

Assistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Conception and design of the study, manuscript preparation.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Conception and design of the study, manuscript preparation.

Assistant Professor, Department of Pathology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Histopathological examinations, manuscript writing.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of Pathology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Histopathological examinations, manuscript writing.

MD, Department of General Surgery, Mengücek Gazi Training and Research Hospital, Erzincan, Turkey. Technical procedures, manuscript preparation. Mengücek Gazi Training and Research HospitalTurkeyErzincan, TurkeyMD, Department of General Surgery, Mengücek Gazi Training and Research Hospital, Erzincan, Turkey. Technical procedures, manuscript preparation.

MD, Department of General Surgery, Zile State Hospital, Tokat, Turkey. Technical procedures, manuscript preparation. Zile State HospitalTurkeyTokat, TurkeyMD, Department of General Surgery, Zile State Hospital, Tokat, Turkey. Technical procedures, manuscript preparation.

MD, Department of General Surgery, Sabuncuoglu Serafettin Training and Research Hospital, Amasya University, Turkey. Technical procedures, manuscript preparation.Amasya UniversityTurkeyTurkeyMD, Department of General Surgery, Sabuncuoglu Serafettin Training and Research Hospital, Amasya University, Turkey. Technical procedures, manuscript preparation.

PhD, Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey. Acquisition, analysis and interpretation of data; technical procedures.Ataturk UniversityTurkeyErzurum, TurkeyPhD, Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey. Acquisition, analysis and interpretation of data; technical procedures.

Assistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Manuscript preparation. Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of General Surgery, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Manuscript preparation.

Assistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Technical procedures, critical revision.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of Anesthesiology and Reanimation, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Technical procedures, critical revision.

Assistant Professor, Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Statistics analysis, manuscript writing, critical revision, final approval.Erzincan Binali Yildirim UniversityTurkeyTurkeyAssistant Professor, Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Turkey. Statistics analysis, manuscript writing, critical revision, final approval.

Figures | Tables

Figures (4)
Tables (2)

Thumbnail

Figure 1 MDA levels in the gastric tissues of SHAM, I/R and TAX+I/R groups. (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group.

Thumbnail

Figure 2 tGSH levels in the gastric tissues of SHAM, I/R and TAX+I/R groups. (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group.

Thumbnail

Figure 3 8-OHdG levels in the gastric tissues of SHAM, I/R and TAX+I/R groups. (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group.

Thumbnail

Figure 4 Histopathological appearance of the gastric tissues of rats in the study groups A. Gastric tissue of SHAM group: normal structures of muscularis mucosa (straight arrow), muscularis propria (round arrow), mucosa (double arrow) and serosa tissues (striped arrow) (HE x100). B. Animal group treated with ischemia-reperfusion in the stomach: destruction and edema of the gastric mucosa (double-sided arrow), partially proliferating dilated congested blood vessel (round arrow), edema (striped arrow) and dilated congested blood vessel in the serosa (straight arrow) (HE x200) 4C. I/R-applied and taxifolin-treated gastric tissue: continuing dilated congested blood vessels in the mucosa (straight arrow) (HE x100).

Thumbnail

Table 1 Biochemical results of the gastric tissues of experimental groups.

Thumbnail

Table 2 Comparisons of histopathological findings of the gastric tissues of experimental groups.

imageFigure 1 MDA levels in the gastric tissues of SHAM, I/R and TAX+I/R groups. (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group. open_in_new

imageFigure 2 tGSH levels in the gastric tissues of SHAM, I/R and TAX+I/R groups. (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group. open_in_new

imageFigure 3 8-OHdG levels in the gastric tissues of SHAM, I/R and TAX+I/R groups. (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group. open_in_new

imageFigure 4 Histopathological appearance of the gastric tissues of rats in the study groups A. Gastric tissue of SHAM group: normal structures of muscularis mucosa (straight arrow), muscularis propria (round arrow), mucosa (double arrow) and serosa tissues (striped arrow) (HE x100). B. Animal group treated with ischemia-reperfusion in the stomach: destruction and edema of the gastric mucosa (double-sided arrow), partially proliferating dilated congested blood vessel (round arrow), edema (striped arrow) and dilated congested blood vessel in the serosa (straight arrow) (HE x200) 4C. I/R-applied and taxifolin-treated gastric tissue: continuing dilated congested blood vessels in the mucosa (straight arrow) (HE x100). open_in_new

table_chartTable 1 Biochemical results of the gastric tissues of experimental groups.

Groups	Oxidant- Antioxidant parameters
Groups	MDA	tGSH	8-OHdG
SHAM	1.83±0.14	4.68±0.17	0.82±0.37
I/R	6.85±0,26*	1.87±0.67*	2.17±0.13*
TAX+I/R	2.38±0.12**	4.20±0.14**	0.99±0.63**

(n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group.

table_chartTable 2 Comparisons of histopathological findings of the gastric tissues of experimental groups.

Groups	Histopathological findings
Groups	Edema	Destruction	Dilated congested blood vessel
SHAM	0 (0-0)	0 (0-0)	0 (0-0)
I/R	3 (3-3) *p<0.001	3 (3-3) *p<0.001	3 (3-3) *p<0.001
TAX+I/R	0 (0-0) **p<0.001	0 (0-0) **p<0.001	1 (1-1) **p=0.41

Data are presented as the median (min-max).
*Compared to SHAM group, ** Compared to IR group, using Kruskal-Wallis and Dunn’s test.

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[TFN1] (n=6), * = p <0.001, according to SHAM group, ** = p <0.001, according to I/R group.

Effect of taxifolin on oxidative gastric injury induced by celiac artery ligation in rats1

Effect of taxifolin on oxidative gastric injury induced by celiac artery ligation in rats¹