Comparison between two types of diluents on the collection and preservation of lowland paca (Cuniculus paca) epididymal spermThe aim of this study was to evaluate the efficiency of two diluents (coconut water powder and milk-based diluent) in preserving sperm of lowland paca. A total of seven animals were subjected to the method for recovering sperm directly from the tail of the epididymis by flotation. The samples obtained directly from the tail of the epididymis had an average volume of 1.5mL, an average concentration of 197.1±84.9 (106 sperm/ml). The average motility and vigor for the coconut water powder (ACP) diluent were 63.8% ± 34.2 and 4.2 ± 1.7 respectively. On the other hand, the samples diluted in milk-based diluent had an average motility of 29.8% ± 34.2 and an average vigor of 2.4 ± 1.9. The membrane integrity of the spermatozoa when diluted with ACP was preserved at 84% ± 0.07 and membrane viability maintained at 53.9% ± 3.78, while the milk-based diluent maintained 73% ± 0.21 of the sperm cells integrated and 39% ± 17.9 of viable membranes. The evaluations of the different diluents indicated that the ACP diluent provided better sperm viability compared to the commercial milk-based diluent. However, both compounds did not preserve the sperm samples for up to 24 hours.
Keywords:
wild animals; biotechnology; sperm refrigeration; epididymal sperm
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