Identification and antimicrobial resistance profile of Enterobacterales and <i>Staphylococcus</i> spp. isolated from subclinical mastitis milk

ALVES, JULIANA S.; SOUZA, ROSSIANE M.; MIRANDA, JENIFFER F.; SILVA, GUILHERME C.L. DA; MOREIRA, JÉSSICA P.L.; GONZALEZ, ALICE G.M.

doi:10.1590/0001-3765202520241287

person JULIANA S. ALVES

Methodology · Data collection · Formal analysis · Investigation · Writing (Original draft preparation) · Writing (Reviewing and Editing).

schoolFluminense Federal University, Faculty of Pharmacy, Department of Bromatology, Hygiene and Food Microbiology Laboratory, Rua Dr. Mário Vianna, 523, 24241-002 Niterói, RJ, Brazil schoolAgricultural Research Company of the State of Rio de Janeiro, State Centre for Research in Animal Health, Biotechnology Laboratory, Alameda São Boaventura, 770, Fonseca, 24120-191 Niterói, RJ, Brazil

0000-0001-9132-9554

person ROSSIANE M. SOUZA

Conceptualization · Funding acquisition · Methodology · Data collection · Supervision · Project administration · Writing (Reviewing and Editing).

schoolAgricultural Research Company of the State of Rio de Janeiro, State Centre for Research in Animal Health, Biotechnology Laboratory, Alameda São Boaventura, 770, Fonseca, 24120-191 Niterói, RJ, Brazil

0000-0001-9807-4291

person JENIFFER F. MIRANDA

Data collection · Formal analysis · Writing (Reviewing and Editing).

schoolAgricultural Research Company of the State of Rio de Janeiro, State Centre for Research in Animal Health, Biotechnology Laboratory, Alameda São Boaventura, 770, Fonseca, 24120-191 Niterói, RJ, Brazil

0000-0002-3749-9381

person GUILHERME C.L. DA SILVA

Data collection · Formal analysis · Writing (Reviewing and Editing).

schoolFluminense Federal University, Faculty of Pharmacy, Department of Bromatology, Hygiene and Food Microbiology Laboratory, Rua Dr. Mário Vianna, 523, 24241-002 Niterói, RJ, Brazil schoolAgricultural Research Company of the State of Rio de Janeiro, State Centre for Research in Animal Health, Biotechnology Laboratory, Alameda São Boaventura, 770, Fonseca, 24120-191 Niterói, RJ, Brazil

0009-0001-7806-5694

person JÉSSICA P.L. MOREIRA

Formal analysis · Writing (Reviewing and Editing).

schoolFluminense Federal University, Faculty of Pharmacy, Department of Bromatology, Hygiene and Food Microbiology Laboratory, Rua Dr. Mário Vianna, 523, 24241-002 Niterói, RJ, Brazil

0000-0003-1987-3584

person ALICE G.M. GONZALEZ

Conceptualization · Funding acquisition · Methodology · Supervision · Writing (Reviewing and Editing).

schoolFluminense Federal University, Faculty of Pharmacy, Department of Bromatology, Hygiene and Food Microbiology Laboratory, Rua Dr. Mário Vianna, 523, 24241-002 Niterói, RJ, Brazil

0000-0001-6789-502X

Correspondence to: Alice G.M. Gonzalez
emailaliceg@id.uff.br

Farm	SCM (%)	MF (%)	Total
A	13 (8.02)	149 (91.98)	162
B	24 (25.00)	72 (75.00)	96
C	7 (7.95)	81 (92.05)	88
Total	44 (12.72)	302 (87.28)	346

Microorganisms	No. of strains (%)						Total
Microorganisms	Farm A		Farm B		Farm C		Total
Enterobacterales	8	(22.22)	3	(15.00)	3	(75.00)	14	(23.33)
Citrobacter braakii	1	(2.78)	0	(0.00)	0	(0.00)	1	(1.67)
Citrobacter freundii	1	(2.78)	0	(0.00)	1	(25.00)	2	(3.33)
Enterobacter asburiae	2	(5.56)	0	(0.00)	0	(0.00)	2	(3.33)
Enterobacter bugandensis	2	(5.56)	1	(5.00)	0	(0.00)	3	(5.00)
Enterobacter cloacae	1	(2.78)	0	(0.00)	0	(0.00)	1	(1.67)
Enterobacter roggenkampii	0	(0.00)	1	(5.00)	2	(50.00)	3	(5.00)
Lelliottia amnigena	1	(2.78)	0	(0.00)	0	(0.00)	1	(1.67)
Serratia marcescens	0	(0.00)	1	(5.00)	0	(0.00)	1	(1.67)
Staphylococcus spp.	28	(77.78)	17	(85.00)	1	(25.00)	46	(76.67)
Staphylococcus aureus	20	(55.56)	15	(75.00)	0	(0.00)	35	(58.33)
Staphylococcus chromogenes	7	(19.44)	2	(10.00)	0	(0.00)	9	(15.00)
Staphylococcus xylosus	1	(2.78)	0	(0.00)	0	(0.00)	1	(1.67)
Staphylococcus saprophyticus	0	(0.00)	0	(0.00)	1	(25.00)	1	(1.67)
Total	36	(60.00)	20	(33.33)	4	(6.67)	60	(100.00)

Microorganisms	No. of non-susceptible strains (%)						Total		No. of MDR strains (%)						Total
Microorganisms	Farm A		Farm B		Farm C		Total		Farm A		Farm B		Farm C		Total
Enterobacterales (n=14)	7	(21.21)	3	(21.43)	3	(100.00)	13	(92.86)	5	(27.78)	0	(0.00)	0	(0.00)	5	(35.71)
Citrobacter braakii (n= 1)	1	(3.03)	0	(0.00)	0	(0.00)	1	(100.00)	1	(5.56)	0	(0.00)	0	(0.00)	1	(100.00)
Citrobacter freundii (n=2)	1	(3.03)	0	(0.00)	1	(33.33)	2	(100.00)	1	(5.56)	0	(0.00)	0	(0.00)	1	(50.00)
Enterobacter asburiae (n=2)	2	(6.06)	0	(0.00)	0	(0.00)	2	(100.00)	1	(5.56)	0	(0.00)	0	(0.00)	1	(50.00)
Enterobacter bugandensis (n=3)	2	(6.06)	1	(7.14)	0	(0.00)	3	(100.00)	2	(11.11)	0	(0.00)	0	(0.00)	2	(66.67)
Enterobacter cloacae (n=1)	1	(3.03)	0	(0.00)	0	(0.00)	1	(100.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)
Enterobacter roggenkampii (n=3)	0	(0.00)	1	(7.14)	2	(66.67)	3	(100.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)
Lelliottia amnigena (n=1)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)
Serratia marcescens (n=1)	0	(0.00)	1	(7.14)	0	(0.00)	1	(100.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)
Staphylococcus spp. (n=46)	26	(78.79)	11	(78.57)	0	(0.00)	37	(80.43)	13	(72.22)	2	(100.00)	0	(0.00)	15	(32.61)
Staphylococcus aureus (n=35)	18	(54.55)	10	(71.43)	0	(0.00)	28	(80.00)	9	(50.00)	1	(50.00)	0	(0.00)	10	(28.57)
Staphylococcus chromogenes (n=9)	7	(21.21)	1	(7.14)	0	(0.00)	8	(88.89)	3	(16.67)	1	(50.00)	0	(0.00)	4	(44.44)
Staphylococcus xylosus (n=1)	1	(3.03)	0	(0.00)	0	(0.00)	1	(100.00)	1	(5.56)	0	(0.00)	0	(0.00)	1	(100.00)
Staphylococcus saprophyticus (n=1)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)	0	(0.00)
Total (n=60)	33	(55.00)	14	(23.33)	3	(5.00)	50	(83.33)	18	(30.00)	2	(3.33)	0	(0.00)	20	(33.33)

Antimicrobial	Farm (%)
Antimicrobial	A		B		C
AMP	5	(62.50)	1	(33.33)	0	(0.00)
AMC	5	(62.50)	2	(66.67)	0	(0.00)
CFZ	5	(62.50)	2	(66.67)	0	(0.00)
CRX	1	(12.50)	1	(33.33)	0	(0.00)
CAZ	0	(0.00)	0	(0.00)	0	(0.00)
CTX	0	(0.00)	0	(0.00)	0	(0.00)
ATM	2	(25.00)	1	(33.33)	3	(100.00)
PPT	0	(0.00)	0	(0.00)	0	(0.00)
TET	0	(0.00)	0	(0.00)	0	(0.00)
MIN	0	(0.00)	0	(0.00)	0	(0.00)
GEN	1	(12.50)	0	(0.00)	0	(0.00)
AMI	0	(0.00)	0	(0.00)	0	(0.00)
NIT	0	(0.00)	1	(33.33)	0	(0.00)
NAL	1	(12.50)	1	(33.33)	3	(100.00)
CIP	0	(0.00)	0	(0.00)	0	(0.00)
LVX	0	(0.00)	0	(0.00)	0	(0.00)
CLO	0	(0.00)	0	(0.00)	0	(0.00)
TRI	1	(12.50)	1	(33.33)	0	(0.00)
SUT	1	(12.50)	1	(33.33)	0	(0.00)
FOS	2	(25.00)	0	(0.00)	0	(0.00)
IPM	0	(0.00)	0	(0.00)	0	(0.00)
MER	0	(0.00)	0	(0.00)	0	(0.00)
ERT	0	(0.00)	0	(0.00)	0	(0.00)

Antimicrobial	Farm (%)
Antimicrobial	A		B		C
PEN	26	(92.86)	11	(64.71)	0	(0.00)
CFO	9	(32.14)	2	(11.76)	0	(0.00)
GEN	0	(0.00)	0	(0.00)	0	(0.00)
ERI	12	(42.86)	2	(11.76)	0	(0.00)
TET	1	(3.57)	0	(0.00)	0	(0.00)
LVX	0	(0.00)	0	(0.00)	0	(0.00)
CIP	0	(0.00)	1	(5.88)	0	(0.00)
NIT	1	(3.57)	1	(5.88)	0	(0.00)
CLI	10	(35.71)	3	(17.65)	0	(0.00)
SUT	4	(14.29)	1	(5.88)	0	(0.00)
TRI	3	(10.71)	2	(11.76)	0	(0.00)
CLO	0	(0.00)	1	(5.88)	0	(0.00)
RIF	7	(25.00)	2	(11.76)	0	(0.00)
LNZ	12	(42.86)	3	(17.65)	0	(0.00)

Microorganisms	Farm (%)						TOTAL
Microorganisms	A		B		C		TOTAL
Staphylococcus spp.	9	(81.82)	2	(18.18)	0	(0.00)	11	(100)
Staphylococcus aureus	7	(77.78)	1	(50.00)	0	(0.00)	8	(72.72)
Staphylococcus chromogenes	2	(22.22)	1	(50.00)	0	(0.00)	3	(27.27)

[tfnI] MF: mastitis-free; SCM: subclinical mastitis. p<0.0001 (p-value of the difference between the number of mammary quarters with SCM and MF among the farms).

Identification and antimicrobial resistance profile of Enterobacterales and Staphylococcus spp. isolated from subclinical mastitis milk