Specific immunohistochemical expression of Mmp-26 in prostatic adenocarcinoma

SILVA, ROMILDO LUCIANO DA; PAES, FRANCISCO LUÍS A.; SILVA, SANDRA MARIA S. DA; SANTOS, FABIANO; SANTANA, EDUARDA S. DE; SILVA NETO, JACINTO DA C.

doi:10.1590/0001-3765202520231380

person ROMILDO LUCIANO DA SILVA

contributed to the conception and design of the study · The collection and processing of the material, as well as the immunohistochemical analyzes · read and approved the final manuscript

schoolUniversidade Federal de Pernambuco, Departamento de Histologia e Embriologia, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50760-420 Recife, PE, Brazil

0000-0001-5680-0902

person FRANCISCO LUÍS A. PAES

contributed to the conception and design of the study · The collection and processing of the material, as well as the immunohistochemical analyzes

schoolUniversidade Federal de Pernambuco, Departamento de Histologia e Embriologia, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50760-420 Recife, PE, Brazil

0000-0003-3130-4296

person SANDRA MARIA S. DA SILVA

contributed to the conception and design of the study · The collection and processing of the material, as well as the immunohistochemical analyzes · read and approved the final manuscript

schoolUniversidade Federal de Pernambuco, Departamento de Histologia e Embriologia, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50760-420 Recife, PE, Brazil

0000-0002-5437-1021

person FABIANO SANTOS

contributed to the conception and design of the study · The collection and processing of the material, as well as the immunohistochemical analyzes · read and approved the final manuscript

schoolGlobal Health Division, IDRC-CRDI, Global Health Division, IDRC-CRDI, 45 O’Connor Street, ON, K1P 1A4, Ottawa, Canada

0009-0004-1951-1991

person EDUARDA S. DE SANTANA

contributed to the conception and design of the study · The revision and editing of the manuscript · read and approved the final manuscript

schoolUniversidade Federal de Pernambuco, Departamento de Histologia e Embriologia, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50760-420 Recife, PE, Brazil

0000-0002-9019-6072

person JACINTO DA C. SILVA NETO

contributed to the conception and design of the study · The analysis of the data and results obtained · The research was supervised by Jacinto Costa da Silva Neto · read and approved the final manuscript

schoolUniversidade Federal de Pernambuco, Departamento de Histologia e Embriologia, Av. Prof. Moraes Rego, 1235, Cidade Universitária, 50760-420 Recife, PE, Brazil

0000-0003-4418-801X

Correspondence to: Jacinto da Costa Silva Neto E-mail:
jacinto.costa@ufpe.br

	Group
Variables (n= 150)	Atrophy (n=25)		NIP (n=25)		HPB (n=50)		ACP (n=50)		p-value
	n	%	n	%	n	%	n	%

Age group (years)									p(¹) = 0.447
41 a 59 (n=27)	3	12	5	20	12	24	7	14
60 a 69 (n= 68)	12	48	11	44	17	34	28	56
70+ (n=55)	10	40	9	36	21	42	15	30
TOTAL	25	100	25	100	50	100	50	100

Ethnicity/color									p(²) = 0.010*
White (n=15)	1	4,8	3	17.6	3	7.0	8	27.6
Brown (n= 66)	9	42.9	9	52.9	30	69.8	18	62.1
Black (n = 29)	11	52.4	5	29.4	10	23.3	03	10.3
TOTAL	21	100	17	100	43	100	29	100

Alcohol consumption									p(¹) = 0.286
Yes (n=44)	7	63.6	3	25.0	17	48.6	17	51.5
No (n=47)	4	36.4	9	75.0	18	51.4	16	48.5
TOTAL	11	100	12	100	35	100	33	100

Smoking									p(¹) = 0.649
Yes (n=49)	9	60	6	42.9	19	50	15	41.7
No (n=54)	6	40	8	57.1	19	50	21	58.3
TOTAL	15	100	14	100	38	100	36	100

			Quantity per group
Variables Exams	Total (n= 150)		Atrophy (n= 25)		NIP (n= 25)		HPB (n= 50)		ACP (n= 50)		p-Value

PSA	n	%	n	%	n	%	n	%	n	%	p(¹) = 0.002*
< 4	37	24.7	06	26.1	06	24	19	47.5	06	12.5
4 to 10	72	48	16	69.6	14	56	16	40	26	54.2
> 10	27	18	01	4.3	05	20	05	12.5	16	33.3
Uninformed	14	9.3

Clinical exam
Presence of nodule	36	24
Absence of nodule	71	47.3
Uninformed	43	28.7

Histopathological
Atrophy	26	17.3
NIP	10	6.7
HPB	29	19.3
ACP	40	26.7
ASAP	02	1.3
Others	08	5.3
Uniformed	35	23.3

Variables		Average ± DP Median (P25; P75)
	Total (n=150)	Atrophy (n=25)	NIP (n=25)	HPB (n=50)	ACP (n=50)	p-Value
Total PSA (n= 136)
	10.68 ± 15.37 5.98 (3.85; 9.13)	5.95 ± 3.12 5.70 (3.90; 7.05)	8.22 ± 8.66 5.62 (3.70; 9.62)	8.81 ± 16.79 4.41 (2.13; 7.26)	15.80 ± 18.90 7.93 (5.46; 18.34)	p(¹) = 0.001*
Free PSA (n= 102)
	1.19 ± 1.13 0.86 (0.50;1.42)	1.05 ± 0.37 0.88 (0.85; 1.43)	1.21 ± 1.36 0.81 (0.40; 1.56)	1.11 ± 1.09 0.82 (0.30; 1.37)	1.30 ± 1.25 0.86 (0.51; 1.74)	p(¹) = 0.628
Free PSA/total PSA (n= 102)
	17.56 ± 10.29 16.33 (10.30; 23.57)	21.20 ± 6.31 22.03 (16.30; 25.29)	19.29 ± 14.64 14.93 (8.27; 28.64)	19.93 ± 9.71 20.17 (15.25; 23.54)	12.85 ± 7.53 11.02 (8.43; 17.00)	p(¹) = 0.001*

			Positive
Group	Negative		Weak		Moderate		Strong		Group Total		p-Value
	n	%	n	%	N	%	n	%	n	%
Atrophy	25	100	-	-	-	-	-	-	25	100	p (¹) < 0,001*
NIP	3	12	22	88	-	-	-	-	25	100
HPB	48	96	2	4	-	-	-	-	50	100
ACP	4	8	10	20	31	62	5	10	50	100
TOTAL	80	53.3	34	22.7	31	20.7	5	3.3	150	100

Variable	Immunohistochemistry						p-Value p	Intensity (IHC)						p-Value
	Total		Negative		Positive			Weak		Moderate		Strong
	n	%	n	%	n	%		n	%	n	%	n	%
Total PSA							p(¹) = 0.002*							p(²) = 0.031*
< 4	37	27.2	27	38.6	10	15.2		5	15.6	5	17.2	-	-
4 to 10	72	52.9	35	50	37	56.1		18	56.3	16	55.2	3	60
> 10	27	19.9	8	11.4	19	28.8		9	28.1	8	27.6	2	40
TOTAL	136	100	70	100	66	100		32	100	29	100	5	100

Clinical examination							p(¹) = 0,945							p(²) = 0,905
Present nodule	36	33,6	18	34	18	33.3		7	30.4	9	33.3	2	50
without nodule	71	66.4	35	66	36	66.7		16	69.6	18	66.7	2	50
TOTAL	107	100	53	100	54	100		23	100	27	100	4	100

[tfnI] (*) Significant difference at the level of 5.0%. (1) Using Pearson’s Chi-square test. (2) Through Fisher’s exact test. In this table, cases without information on each variable were not considered.