We provide initial findings on reproductive performance of the poorly known killifish, Anablepsoides ornatus, from the Rio Negro basin. We recorded fecundity, spawning frequency, egg diameter, fertilization rate, duration of embryonic development, and hatching success from the F1 generation for 92 days. Additionally, we evaluated the fish preferences for either green or black spawning substrates. Four spawning pairs produced a total of 53 eggs (diameter = 1,428.0±98.5 µm). The average fertilization and hatching rate ranged from 54.7 to 87.8% among pairs. The preference for the black substrate was significantly higher. The duration of embryonic development ranged from 16 to 72 days, and the interval between spawnings ranged from 6.5 to 14.5 days among pairs. Our findings provide a baseline for future studies on the ecology of the species, thereby enhancing our understanding of its reproductive biology, informing conservation practices and the potential for culturing in the ornamental fish trade.
KEYWORDS:
non-annual rivulid; reproductive performance; spawning substrate
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